June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Evaluation of Ocular Injection Quality in Rodents, Rabbits, and Large Species
Author Affiliations & Notes
  • Glenwood G Gum
    Ophthalmology, Pharmaron(US) Lab Services, San Diego, California, United States
  • Sandeep Kumar
    Ophthalmology, Pharmaron(US) Lab Services, San Diego, California, United States
  • Footnotes
    Commercial Relationships   Glenwood Gum Pharmaron (US) Lab Services, Code E (Employment); Sandeep Kumar Pharmaron(US) Lab Servicess, Code E (Employment)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3708. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Glenwood G Gum, Sandeep Kumar; Evaluation of Ocular Injection Quality in Rodents, Rabbits, and Large Species. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3708.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Precise ocular drug delivery has been a challenge for researchers either due to the nature of a drug compound, complex delivery routes or an untrained surgeon performing delivery routes like intravitreal (IVT), subretinal (SR) and suprachoroidal (SC) in animals. Mis-doses may result in either low or no ocular bioavailability, which poses a financial burden to academic and industry researchers. Therefore, we have evaluated the success rate of IVT, SR and SC routes by delivering implants, viral vectors and cell therapy products in rodents, rabbits, dogs, and pigs.

Methods : Mice (C57BL6, N=100) and rats (Nude and RCS; N=150) for IVT and SR; Rabbits (N=100) for IVT and SC; Dogs and pigs (N=25) for IVT and SC injections were included. Injection quality was evaluated immediately post injections using color fundus, optical coherence tomography (OCT), slit lamp biomicroscopy and indirect ophthalmoscopy. Parameters like retinal trauma, retinal/vitreous/scleral hemorrhage, and lens damage were evaluated. Experienced and newly trained surgeons performed the injections and results were compared.

Results : IVT injection success rate was 100% when done by an experienced surgeon, regardless of the species or type of drug product. Rat SR injection rate was dependent on strain or type of therapy, like cells vs virus. In nude rats, during SR injection of cells, occasional lens damage and slight to moderate vitreous hemorrhage were observed. SC injection success rate was dependent on the type of delivery device used. Customized device has ~70% success rate. Scleral hemorrhage and SR or IVT mis-dose was observed rarely during SC injections. Injection success rate was lower with newly trained surgeons (Table.1).

Conclusions : In vivo imaging shall be performed immediately after delivery to evaluate the injection quality. Animals with poor injections shall be excluded and replaced with an animal with good injection. Our results suggest that a good training program and significant hands on experience are necessary for the successful delivery of ocular compounds via these specialized routes of delivery.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×