June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Composition and comparison of core conjunctival sac microbiome in Hong Kong Children by age, sex and ethnicities
Author Affiliations & Notes
  • Xiangtian Ling
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Xiu Juan Zhang
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Christine Bui
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Clement C. Tham
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Calvin C P Pang
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Li Jia Chen
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Jason C. Yam
    The Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Footnotes
    Commercial Relationships   Xiangtian Ling None; Xiu Juan Zhang None; Christine Bui None; Clement Tham None; Calvin Pang None; Li Jia Chen None; Jason C. Yam None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 204. doi:
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      Xiangtian Ling, Xiu Juan Zhang, Christine Bui, Clement C. Tham, Calvin C P Pang, Li Jia Chen, Jason C. Yam; Composition and comparison of core conjunctival sac microbiome in Hong Kong Children by age, sex and ethnicities. Invest. Ophthalmol. Vis. Sci. 2023;64(8):204.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To identify the core microbial community in conjunctival sac of Hong Kong healthy children using 16S rRNA next generation sequencing and to assess the factors influencing the compositions of the conjunctival microbiome.

Methods : Healthy children of different ethnicities (Chinese, India, Pakistani, Nepalese, Filipino) in Hong Kong were included and conjunctival swabs were taken bilaterally. DNA was extracted and sequenced on an Illumina platform. Amplicon sequence variants (ASVs) were generated using DADA2 in Qiime2 and analyzed with MicrobiomeAnalyst.

Results : A total of 196 conjunctival samples from 102 children (mean age ± SD, 8.9 ± 2.5 years; 44% female) were analyzed. Staphylococcus, Streptococcus, Cutibacterium, Corynebacterium, Enhydrobacter were found to be the most abundant genera. There were no significant age (4-9 and 10-15 years) or gender differences in the microbial compositions and alpha diversities. Among all ethnicities, Filipino children had the highest alpha diversity. Linear discriminant analysis found 9 genera (Corynebacterium, Escherichia_Shigella, Cuibacterium, Micrococcus, Streptococcus, Enhydrobacter, Dolosigranulum, Gemella, Acinetobacter) to be significantly different among different ethnicities.

Conclusions : Our results identify the core microbial community of ocular surface in Hong Kong children and suggest that there are differences in the microbiome among children of different ethnicities who live in the same city. Further metagenomic study is required to how these compositional differences contribute to function of microbiome.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

 

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