June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Visible-light optical coherence tomography fibergraphy (vis-OCTF) in patients with optic neuropathies
Author Affiliations & Notes
  • Hao Zhang
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Weijia Fan
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • David Andrew Miller
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Avram Bar Meir
    Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Arnold Nadel
    Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Xiaorong Liu
    University of Virginia, Charlottesville, Virginia, United States
  • Rukhsana Mirza
    Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Nicholas J Volpe
    Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Shira Simon
    Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Hao Zhang Opticent Inc., Code I (Personal Financial Interest); Weijia Fan None; David Miller None; Avram Bar Meir None; Arnold Nadel None; Xiaorong Liu None; Rukhsana Mirza None; Nicholas Volpe Opticent Inc., Code I (Personal Financial Interest); Shira Simon None
  • Footnotes
    Support  NIH R01EY029121, U01EY033001, R44EY026466
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1967. doi:
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      Hao Zhang, Weijia Fan, David Andrew Miller, Avram Bar Meir, Arnold Nadel, Xiaorong Liu, Rukhsana Mirza, Nicholas J Volpe, Shira Simon; Visible-light optical coherence tomography fibergraphy (vis-OCTF) in patients with optic neuropathies. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1967.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To achieve mapping of individual retinal ganglion cell (RGC) fiber bundles in humans and quantifying RGC fiber bundle properties to be potentially correlated with the type and clinical course of optic neuropathy.

Methods : We developed visible-light optical coherence tomography (vis-OCT) for this goal. This vis-OCT system used a dual-spectrometer balanced-detection architecture to achieve shot-noise limited imaging at 125-kHz A-line rate. The maximum field of view (FOV) is 56 degrees, the axial resolution is 1.3 µm, and the later resolutions are 7 µm at the central FOV and 12 µm at the peripheral FOV. After acquiring a three-dimensional vis-OCT volume, we performed image registration to flatten the retina and isolated individual RGC fiber bundles in the segmented retinal nerve fiber layer (RNFL). We also developed a digital resampling method to obtain speckle-reduced B-scan images along arbitrary trajectories on the fundus to better visualize the cross-sections of individual RGC fiber bundles.

Results : We imaged 18 patients with different types and severity of optic neuropathy and failed to obtaine high-quality images from four patients due to the severely-degraded optical quality. In five patients, we focused on visualizing individual RGC fiber bundles near the temporal raphe. From a 3×3 mm2 area, we mapped the RGC bundle density, orientation, and individual bundle cross-sectional areas. Taking the macula as the pole of a polar coordinate, vis-OCTF revealed an angle-dependence of the RGC bundle height, width, and cross-sectional area, where the bundle height and cross-sectional area increase more dramatically than the width with angular deviation from the temporal raphe. In this pilot study, we also observed a trend that patients with compressive optic neuropathy showed much enlarged RGC bundle width and slightly enlarged RGC bundle height; however, further studies are needed to reach a statistically significant conclusion.

Conclusions : Our vis-OCT achieved high-quality imaging at 125-kHz A-line rate in patients without severe cataracts. Vis-OCTF mapped individual RGC fiber bundles around the temporal raphe and measured the angle-dependence of bundle width, height, and cross-sectional areas with respect to the temporal raphe. Vis-OCTF has the potential to improve our understanding and clinical management of optic neuropathy.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

(a) Individual RGC fiber bundles. (b) Resampled B-scan image.

(a) Individual RGC fiber bundles. (b) Resampled B-scan image.

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