June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Visible light Optical Coherence Tomography imaging of the myoid-ellipsoid boundary in the mouse
Author Affiliations & Notes
  • Pooja Chauhan
    Radiology, NYU Langone Health, New York, New York, United States
  • Aaron M Kho
    Biomedical Engineering, University of California Davis, Davis, California, United States
  • Vivek Srinivasan
    Radiology and Ophthalmology, NYU Langone Health, New York, New York, United States
  • Footnotes
    Commercial Relationships   Pooja Chauhan None; Aaron Kho None; Vivek Srinivasan Optovue, Inc, Code P (Patent)
  • Footnotes
    Support  National Eye Institute Grants R01EY031469 (to V.J.S.), R01NS094681 (to V.J.S.), T32EY015387 (National Eye Institute UC Davis training grant), and P30EY012576 (National Eye Institute UC Davis core grant) and Research to Prevent Blindness Unrestricted Grant (to NYU Langone Health Department of Ophthalmology).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2920. doi:
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    • Get Citation

      Pooja Chauhan, Aaron M Kho, Vivek Srinivasan; Visible light Optical Coherence Tomography imaging of the myoid-ellipsoid boundary in the mouse. Invest. Ophthalmol. Vis. Sci. 2023;64(8):2920.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Here, we use visible light Optical Coherence Tomography (OCT) to investigate the inner segment (IS) myoid (“m”) and ellipsoid (“e”) zones in the C57BL/6J mouse retina.

Methods : A free-space visible light spectral / Fourier domain OCT system was used for in vivo retinal imaging of C57BL/6J (n=11, 9.87 ± 7.92 months, 9 males, 2 females) mice from The Jackson Laboratory. The full spectral width used for imaging was 259 nm and the axial resolution was 1.0 μm in tissue. Eight repeated volumetric datasets with 512 a-lines and 128 b-scans each were acquired with a 1 mm fast axis range and a 0.12 mm slow axis range, for speckle reduction. Each volumetric dataset was acquired in 17.5 seconds. Raw OCT data were motion-corrected and averaged along the slow axis to yield high-quality cross-sectional images (Figure 1A). The region near the ELM was fitted at each transverse location in 2x upsampled, background-corrected line profiles. A Gaussian plus offset model was employed to represent the ELM and surrounding (outer nuclear layer and IS) reflectivity, respectively (Figure 1B). Line profiles with R-squared > 0.91 and Gaussian (ELM) standard deviation < 4 pixels were retained, normalized to the offset, and displayed as an image (Figure 1C). All retained line profiles for each data set were averaged, yielding relative intensity profiles.

Results : We observed a subtle reflectivity division in the IS, which was revealed to consist of a more reflective, “m” zone, and a less reflective, “e” zone (Figure 1C). The division was present to some degree in both younger and older mice (Figure 1D).

Conclusions : Our results show that visible light OCT can distinguish a subtle IS reflectivity division in the mouse. Findings were consistent with prior human OCT study (Srinivasan et al., TVST 2022) which showed the myoid zone to be more reflective than the inner ellipsoid, and cytochrome C immunostaining in the mouse (Cuenca et al., Prog. Retin. Eye Res. 2020) which shows a myoid on the order of 5 microns in length.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

Figure 1. (A) Visible light OCT image of a 19 month old mouse retina. (B) OCT intensity profile with fit (Gaussian plus offset, R-squared=0.97) to determine ELM location. (C) outer ONL and IS zoom with ELM flattening and ONL normalization depicts a reflectivity division within the IS. (D) Average relative intensity in young and old mice as a function of depth, measured outer to the ELM (mean ± std. dev.).

Figure 1. (A) Visible light OCT image of a 19 month old mouse retina. (B) OCT intensity profile with fit (Gaussian plus offset, R-squared=0.97) to determine ELM location. (C) outer ONL and IS zoom with ELM flattening and ONL normalization depicts a reflectivity division within the IS. (D) Average relative intensity in young and old mice as a function of depth, measured outer to the ELM (mean ± std. dev.).

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