June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Changes in choroidal angioarchitecture after laser photocoagulation (LP) induced choroidal neovascularisation (CNV) in non-human primates (NHPs)
Author Affiliations & Notes
  • Eunice Jin Hui Goh
    National Healthcare Group, Singapore, Singapore
  • Veluchamy Barathi
    Singapore Eye Research Institute, Singapore, Singapore
  • Rupesh Vijay Agrawal
    Tan Tock Seng Hospital, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Eunice Jin Hui Goh None; Veluchamy Barathi None; Rupesh Agrawal None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1791. doi:
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      Eunice Jin Hui Goh, Veluchamy Barathi, Rupesh Vijay Agrawal; Changes in choroidal angioarchitecture after laser photocoagulation (LP) induced choroidal neovascularisation (CNV) in non-human primates (NHPs). Invest. Ophthalmol. Vis. Sci. 2023;64(8):1791.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : CNV secondary to age-related macular degeneration (AMD) is the leading cause of blindness especially in the elderly. The mechanism of AMD involves complex risk factors involving the Bruch’s membrane, retinal pigment epithelium and choroidal structures. Studies have been divided on its effect on choroidal thickness, often used as a parameter to evaluate choroidal vasculature abnormalities. These were based on patients with AMD, subject to confounding factors such as disease severity, lifestyle and treatment compliance. In our study performed on healthy NHPs, we sought to eliminate these factors by recapitulating the main features of exudative AMD with LP and evaluating these eyes instead.

Methods : We evaluated changes in choroidal angioarchitecture on optical coherence tomography (OCT) post-LP in NHPs using the choroidal vascularity index (CVI), a sensitive method that detects changes in choroidal angioarchitecture.
CNV was induced by LP on Day 0 in both eyes of 8 cynomolgus monkeys. Laser-induced lesions were confirmed and severity scored using fluorescein angiography from grade 1 (no hyperfluorescence) to grade 4 (bright hyperfluorescence and leakage) by a masked investigator on Day 14 and continued till Day 28. Enhanced depth imaging OCTs were also performed simultaneously and segmented by modified image binarization technique. The CVI for each eye and overall change in both eyes were calculated.

Results : Out of the 16 eyes, 14 were noted to have a reduction in CVI at day 28 compared to baseline. 2 NHPs had 1 eye each which showed a converse increase in vascularity, but both had an overall reduction in CVI when both eyes were included in the calculation (mean of -5.59% in all 8 NHPs).

It was interesting to note that on day 14 of assessment, 6 out of 16 eyes showed this converse increase in vascularity but by day 28 only 2 of them still maintained this increase in vascularity compared to baseline. We postulated that this could be due to vasodilation induced by choroidal obstruction or effusion in the early days post-LP.

Conclusions : This study evaluates the effect of CNV secondary to wet AMD on the choroidal vasculature in healthy NHPs, eliminating potential confounding factors in human subjects. It revealed that in most eyes, PRP led to a reduction in CVI by day 28.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

Sample of an analysed image at baseline (top), day 14 (middle) and day 28 (bottom)

Sample of an analysed image at baseline (top), day 14 (middle) and day 28 (bottom)

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