June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Effects of glaucoma and optic nerve crush on the biomechanical behavior of mouse astrocytic lamina cribrosa
Author Affiliations & Notes
  • Arina Korneva
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Elizabeth Kimball
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Thomas Johnson
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Sarah Quillen
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Mary E Pease
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Thao Nguyen
    Mechanical Engineering, Johns Hopkins University Whiting School of Engineering, Baltimore, Maryland, United States
  • Harry A Quigley
    Ophthalmology, Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Arina Korneva None; Elizabeth Kimball None; Thomas Johnson None; Sarah Quillen None; Mary Pease None; Thao Nguyen None; Harry Quigley None
  • Footnotes
    Support  NIH Grant R01EY02120, NIH Grant P30EY01765
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 4365. doi:
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      Arina Korneva, Elizabeth Kimball, Thomas Johnson, Sarah Quillen, Mary E Pease, Thao Nguyen, Harry A Quigley; Effects of glaucoma and optic nerve crush on the biomechanical behavior of mouse astrocytic lamina cribrosa. Invest. Ophthalmol. Vis. Sci. 2023;64(8):4365.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare the astrocytic lamina (AL) without damage, after optic nerve crush (ONC), and in experimental glaucoma in the magnitude of deformation due to short-term inflation.

Methods : Adult mice, of both sexes, with enhanced GFP fluorescence widespread in all cells (heterozygous from C57BL/6-Tg(CAG-EGFP)1OSb/J Jackson #003291) were used. Unilateral ONC was performed in one group, unilateral injection of microbeads (experimental glaucoma) was performed in another group. Mice were euthanized 3 days and 6 weeks later and their eyes inflation-tested; controls were untreated fellow eyes. Intraocular pressure (IOP) was increased by anterior chamber cannulation in explanted mouse eyes, the AL was imaged with two-photon laser scanning microscopy, and the deformation and strain in the astrocytic lamina were calculated as described previously (Korneva et al., 2020). Axon loss was estimated from optic nerve cross-sections.

Results : Nasal-temporal (Exx), superior-inferior (Eyy), shear (Exy), anterior-posterior (Ezz), and the 3 principal strains were calculated. Strains were averaged across the AL. Strains of specimens 3 days or 6 weeks of experimental glaucoma or ONC were not statistically different from strains of controls. Strains were also averaged across the central AL or across the peripheral AL (see Korneva et al., 2020). Exx and Eyy in the central, were greater 3 days after microbead injection than in control (Exx=0.090±0.047 vs. 0.029±0.066, p=0.049; Eyy=0.055±0.037 vs. 0.023±0.021, p=0.049; n=8 each), but not in the peripheral AL. Specimen-averaged and central strains in glaucoma specimens were not significantly related to IOP exposure levels. In specimens with 6 weeks of glaucoma or ONC, central and peripheral strains were indistinguishable from controls (all p>0.37; n=8 and 16) and were not significantly different between central and peripheral zones in either group.

Conclusions : We confirmed a short-term, dynamic change in AL mechanical behavior in experimental glaucoma eyes 3 days after microbead injection, but found no similar strain change after ONC. Regional strains in the central compared to peripheral AL in experimental glaucoma suggest local alterations in astrocyte responses.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

Nasal-temporal (Exx) and superior-inferior (Eyy) strain, and principal strains (Emax, Emin, γmax) averaged across the central AL. Mean±STD

Nasal-temporal (Exx) and superior-inferior (Eyy) strain, and principal strains (Emax, Emin, γmax) averaged across the central AL. Mean±STD

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