June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Measurements of light-evoked photoreceptor function in diseased retinas using proto-clinical optoretinography
Author Affiliations & Notes
  • Christopher S Langlo
    Dept. of Ophthalmology and Vision Science, University of California Davis, Sacramento, California, United States
  • Reddikumar Maddipatla
    Dept. of Ophthalmology and Vision Science, University of California Davis, Sacramento, California, United States
    EyePod Imaging Lab, Dept. of Cell Biology and Human Anatomy, University of California Davis, Davis, California, United States
  • Kari V Vienola
    Institute of Biomedicine, Turun yliopisto, Turku, Varsinais-Suomi, Finland
  • Robert J Zawadzki
    Dept. of Ophthalmology and Vision Science, University of California Davis, Sacramento, California, United States
    EyePod Imaging Lab, Dept. of Cell Biology and Human Anatomy, University of California Davis, Davis, California, United States
  • Ravi Sankar Jonnal
    Dept. of Ophthalmology and Vision Science, University of California Davis, Sacramento, California, United States
  • Footnotes
    Commercial Relationships   Christopher Langlo None; Reddikumar Maddipatla None; Kari Vienola Filed patent application 5/22 for clinical ORG, Code P (Patent); Robert Zawadzki Filed patent application 5/22 for clinical ORG, Code P (Patent); Ravi Jonnal Filed patent application 5/22 for clinical ORG, Code P (Patent)
  • Footnotes
    Support   NEI grants R01EY031098, R01EY026556, R01EY033532, and P30EY012576
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1360. doi:
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    • Get Citation

      Christopher S Langlo, Reddikumar Maddipatla, Kari V Vienola, Robert J Zawadzki, Ravi Sankar Jonnal; Measurements of light-evoked photoreceptor function in diseased retinas using proto-clinical optoretinography. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1360.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : While recent decades have brought immense advances in assessment of retinal structure with optical coherence tomography (OCT), noninvasive objective functional assays remain elusive. Electroretinography provides functional information but lacks fine anatomic localization, and requires the use of electrodes. Here we demonstrate use of OCT-based optoretinography (ORG) without adaptive optics to provide localized noninvasive light-evoked functional information in diseased retinas.

Methods : A previously described clinical grade OCT system was used to obtain images in normal and diseased retinas after dark adaptation. An LED flash was synced with OCT acquisition. B-scans were obtained at 400Hz at either two or six degrees from the foveal center. Resulting B-scans were flattened to align the cone inner segment/outer segment junction (IS/OS) and cone outer segment tip (COST) bands. Relative phase velocities of IS/OS and COST were computed in a rolling window, pre and post-flash, and then stimulus-evoked OS length changes were measured. Multiple measures were made in each image set. Research was conducted in compliance with the Declaration of Helsinki. Three patients with retinal disease were recruited for imaging; one each with retinitis pigmentosa, ABCA-4 related macular dystrophy and age-related macular degeneration. The ORG responses were compared to those of a healthy retina.

Results : Measurable responses were seen in all retinas but the magnitude was decreased in the eyes with retinal disease. The outer retinal layer disruption in these disease states lead to smaller areas of retina used to generate the ORG signal in these images compared to normal control (see figure).

Conclusions : We have shown the feasibility of using OCT to gain functional data directly linked to structural imaging in human diseased retinas. Limitations arose due to structural degeneration; these will be addressed by different acquisition strategies in future imaging. This technique could be useful for clinical management of retinal disease as well as monitoring retinal function in clinical trials. Further refinement of the technique is needed to understand how best to measure the ORG response in disease states using clinical-grade OCT systems.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

B-scan images of normal (A) and RP (B) retinas, with outlines over areas of ORG signal measurement. ORG traces seen to the right, black lines represent averaged traces.

B-scan images of normal (A) and RP (B) retinas, with outlines over areas of ORG signal measurement. ORG traces seen to the right, black lines represent averaged traces.

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