Abstract
Purpose :
Freshly harvested bovine eyeballs were injected intracamerally and intravitreally with DATTS and incubated in oxygenated Krebs buffer solution (37°C) for up to 6 h. Cornea, lens, aqueous humor (AH), iris-ciliary body (ICB), vitreous humor (VH) and retina were dissected hourly, homogenized and the supernatant was analyzed for H2S content potentiometrically using WPI ISO-H2S-100.
Methods :
Freshly harvested bovine eyeballs were injected intracamerally and intravitreally with DATTS and incubated in oxygenated Krebs buffer solution (37°C) for up to 6 h. Cornea, lens, aqueous humor (AH), iris-ciliary body (ICB), vitreous humor (VH) and retina were dissected hourly, homogenized and the supernatant analyzed for H2S content potentiometrically using WPI ISO-H2S-100.
Results :
Endogenous H2S content in cornea, lens, AH, ICB, VH, and retina of untreated eyeballs (control; n=3) was 47±5, 127±11, 39±4, 43±3, 27±2, 41±2 nmoles/gram of tissue, respectively. Following intravitreal injection of DATTS (10-9M, 50µL), a significant (p<0.001; n=3), maximal increase in H2S content was achieved after 2 h in cornea (156±11%), AH (90±7%), ICB (114±7%), and VH (107±13%). Interestingly, H2S content attained the highest increase (260±18%(p<0.001) in the lens after only 1 h of incubation. On the other hand, intracameral injection of DATTS (10-9M, 50µL) significantly (n=3; p<0.001) increased H2S content in cornea (130±17%), AH (231±25%) and lens (266±20%) after an incubation period of 6 h. Interestingly, there was a significant (p<0.001; n=3) reduction in H2S content in ICB (to 30±1%; and retina (to 77±0.7%) after intracameral injection
Conclusions :
We conclude that intravitreally-administered DATTS rapidly increases H2S concentrations in various ocular tissues, whereas intracameral injection failed to increase the concentration of H2S in the posterior segment structures of the eye. It appears that intravitreal injections provide a more versatile route for the administration of H2S-releasing compounds in the eye.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.