June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
A novel three-dimensional imaging and assessment of the New Zealand White rabbit eye
Author Affiliations & Notes
  • Stefan Linehan
    TissueVision Inc, Newton, Massachusetts, United States
  • Lydia Andrews-Jones
    AbbVie Inc, North Chicago, Illinois, United States
  • Gianna Ferron
    TissueVision Inc, Newton, Massachusetts, United States
  • Ronnie Nie
    AbbVie Inc, North Chicago, Illinois, United States
  • John Masterson
    AbbVie Inc, North Chicago, Illinois, United States
  • Jie Shen
    AbbVie Inc, North Chicago, Illinois, United States
  • Joshua Rowe
    AbbVie Inc, North Chicago, Illinois, United States
  • Tim Ragan
    TissueVision Inc, Newton, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Stefan Linehan None; Lydia Andrews-Jones None; Gianna Ferron None; Ronnie Nie None; John Masterson None; Jie Shen None; Joshua Rowe None; Tim Ragan None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 5026. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Stefan Linehan, Lydia Andrews-Jones, Gianna Ferron, Ronnie Nie, John Masterson, Jie Shen, Joshua Rowe, Tim Ragan; A novel three-dimensional imaging and assessment of the New Zealand White rabbit eye. Invest. Ophthalmol. Vis. Sci. 2023;64(8):5026.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : When attempting to evaluate and understand ocular pathologic processes, histologic methods allow the most detailed morphologic evaluation of ocular changes but are limited to two-dimensional (2D) imaging. There is great benefit for many questions to be able to evaluate the full, intact structure of the eye with three-dimensional (3D) modeling. Here we report a novel ex vivo 3D assessment of the New Zealand White rabbit eye using the TissueVision, Inc. (TVI) TissueCyte Serial Two-Photon Plus (STP2) imaging platform.

Methods : Fixed rabbit eye samples were embedded in an agarose block and polymerized in an embedding matrix to provide stability for 100 micron-thick sectioning. Samples were imaged using two-photon excitation and targeted regions of interest were assessed. Gross tissue anatomy was visualized via autofluorescence, and collagen signatures of the cornea and sclera were visualized via second harmonic generation (SHG) imaging.

Results : High-resolution fully aligned datasets were produced for each eye. Targeted regions of muscle, retina, iris, ciliary body, and lens were visualized via autofluorescence. An assessment of collagen in the cornea and sclera revealed distinctive collagen fibril bundle organization.

Conclusions : The ability to process intact New Zealand White rabbit eyes using the STP2 imaging platform demonstrates a novel method to produce a 3D model of total gross morphology and collagen signatures of the cornea and sclera. This finding elucidates great potential for the future evaluation of disorders of the eye.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×