To investigate the effects of different optical conditions on choroidal thickness over time, a two-factor repeated measures analysis of variance (ANOVA) was conducted. The factors examined were “Optical Conditions” (with four levels: NoAO-Myopia, NoAO-Hyperopia, AO-Myopia, and AO-Hyperopia) and “Time” (with seven levels: 0, 10, 20, 30, 34, 38, and 45 minutes). After conducting the repeated measures ANOVA, we performed pairwise comparisons using Tukey's post hoc analysis. This analysis allowed us to examine the specific differences in ChT at different time points compared to the baseline for each optical condition (where time was treated as a fixed factor). Additionally, we assessed the differences between optical conditions for each time point during the exposure phase (treating optical conditions as a fixed factor). All statistical analyses were conducted using SPSS version 26 (IBM Corp., Armonk, NY, USA). The statistical significance level was set at P < 0.05 to determine the presence of significant differences.