Abstract
Purpose :
MALT lymphoma orbital region and IgG4-related eye disease (IGG4ROD) account for the majority of mass lesions in the orbital region. They may show similar shadows on images, and many cases are difficult to differentiate clinically. We investigated what kind of gene expression cluster differences are responsible for the origin of the two groups of diseases.
Methods :
Methods: Total RNA was extracted from fresh tissues of ８cases of orbital MALT lymphoma and 11cases of orbital IgG4-related eye disease, which were histopathologically diagnosed from 20XX to 20XX, and a sequence library was created. Next-generation sequencing analysis (RNA-seq, NovaSeq6000) was performed to compare gene expression between the two groups, and principal component analysis and cluster hierarchy analysis were performed.
Results :
n principal component analysis, orbital MALT lymphoma and IgG4ROD were clustered in component 2.
Clear cluster differences were observed, except for one case that appeared to be IgG4-producing MALT lymphoma. In addition, cluster analysis showed that one case that appeared to be IgG4-producing MALT lymphoma was located just between the IGG4ROD and MALT lymphoma clusters. Furthermore, in a comparison of up regulator analysis between IgG4ROD and MALT lymphoma, 582 genes were identified as candidates, including ETV6-RUNX1 and Sting1 (P<0.01). Pathway analysis of MALT lymphoma suggested that the Sting-CCL22 pathway may be activated.
Conclusions :
MALT lymphoma orbital and IgG4-related eye disease (IGG4ROD) can be divided into two clusters of gene expression, and IgG4-producing MALT is located in the border region. The Sting-CCL22 pathway may be a proliferation pathway specific to orbital MALT lymphoma.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.