Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
DEVELOPMENT OF A MICRONEEDLE FOR INTRAOPERATIVE CANNULATION OF RETINAL VESSELS
Tongalp H Tezel; Aykut Aksit; David Koenigstein; Ahmet Hondur; Anil K Lalwani; Jeffrey W Kysar
Author Affiliations & Notes
  • Tongalp H Tezel
    Department of Ophthalmology, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Aykut Aksit
    Fu Foundation School of Engineering & Applied Science, Mechanical Engineering, Columbia University, New York, New York, United States
    Department of Otolaryngology-Head and Neck Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • David Koenigstein
    Department of Ophthalmology, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Ahmet Hondur
    Department of Ophthalmology, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Anil K Lalwani
    Department of Otolaryngology-Head and Neck Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Jeffrey W Kysar
    Fu Foundation School of Engineering & Applied Science, Mechanical Engineering, Columbia University, New York, New York, United States
    Department of Otolaryngology-Head and Neck Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Footnotes
    Commercial Relationships   Tongalp Tezel None; Aykut Aksit Haystack Medical, Inc, Code O (Owner); David Koenigstein None; Ahmet Hondur None; Anil Lalwani Spiral Therapeutics, Code C (Consultant/Contractor), Haystack Medical, Inc, Code O (Owner); Jeffrey Kysar Haystack Medical, Inc, Code O (Owner)
  • Footnotes
    Support  Supported by an unrestricted grant from Research to Prevent Blindness, Inc., New York (THT), NY, Foley Research Fund, New York, NY (THT), and Discretionary Research Fund (AKL).
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 895. doi:
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      Tongalp H Tezel, Aykut Aksit, David Koenigstein, Ahmet Hondur, Anil K Lalwani, Jeffrey W Kysar; DEVELOPMENT OF A MICRONEEDLE FOR INTRAOPERATIVE CANNULATION OF RETINAL VESSELS. Invest. Ophthalmol. Vis. Sci. 2024;65(7):895.

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Abstract

Purpose : To develop a microneedle for intraoperative cannulation of the retinal vessels.

Methods : Two-photon polymerization lithography was employed to fabricate microneedles (80 μm outer diameter/60 μm inner diameter) with ultrasharp tips and curved lumina. Several different designs were tested for the ease of entering the retinal vessels using freshly enucleated (< 12 hours) pig eyes. Design modifications were made to puncture the retinal vessels in a minimally damaging fashion and increase their cannulation ability.

Results : An 80 μm microneedle (60 mm inner diameter) with a curved tip was the most suitable microneedle to puncture the retinal vessels. (Figure 1a) It allowed the surgeon to visualize the tip of the needle and correctly position it over the retinal vessels. A serrated blade-like manufacturing of the microneedle’s edge allowed it to slice the upper vessel wall along the direction of the vessel wall fiber. Wing-like extensions were added to the design to align the needle tip with the vessel that will be cannulated and limit the insertion depth of the needle, thus preventing the perforation of the retinal vessel wall. Using the finalized design, retinal vessels were entered and perfused successfully. (Figure 1 b, c)

Conclusions : The novel retinal microneedle design allows for perfusion of the retinal vessels. This transformative technology has the potential to make possible treatment of several retinal diseases including, retinal vascular occlusions, subretinal gene therapy, removal of subretinal perfluorocarbon droplets as well as selective chemotherapy of retinal malignancies.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

 

(A) Schematics of the retinal microneedle. Curved entry allows the surgeon to visualize the tip of the needle as it enters the vessel. Wings align the tip and prevent perforation of the vessel wall; (B) Needle approaching the retinal venule during the ex vivo experiment; (C) BSS is injected into the vessel after having access to its lumen. The blood column in the retinal vessels moves as it is perfused
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(A) Schematics of the retinal microneedle. Curved entry allows the surgeon to visualize the tip of the needle as it enters the vessel. Wings align the tip and prevent perforation of the vessel wall; (B) Needle approaching the retinal venule during the ex vivo experiment; (C) BSS is injected into the vessel after having access to its lumen. The blood column in the retinal vessels moves as it is perfused

(A) Schematics of the retinal microneedle. Curved entry allows the surgeon to visualize the tip of the needle as it enters the vessel. Wings align the tip and prevent perforation of the vessel wall; (B) Needle approaching the retinal venule during the ex vivo experiment; (C) BSS is injected into the vessel after having access to its lumen. The blood column in the retinal vessels moves as it is perfused

(A) Schematics of the retinal microneedle. Curved entry allows the surgeon to visualize the tip of the needle as it enters the vessel. Wings align the tip and prevent perforation of the vessel wall; (B) Needle approaching the retinal venule during the ex vivo experiment; (C) BSS is injected into the vessel after having access to its lumen. The blood column in the retinal vessels moves as it is perfused
View OriginalDownload Slide

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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