Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Ultrasound Biomarkers: Contrast Enhanced Ultrasound and Nakagami Imaging to Differentiate Benign and Malignant Choroidal Tumor
Author Affiliations & Notes
  • Vishal Raval
    LV Prasad Eye Institute, Hyderabad, Telangana, India
  • Karla P. Mercado-Shekhar
    Indian Institute of Technology Gandhinagar, Gandhinagar, Gujarat, India
  • Himanshu Shekhar
    Indian Institute of Technology Gandhinagar, Gandhinagar, Gujarat, India
  • Footnotes
    Commercial Relationships   Vishal Raval None; Karla P. Mercado-Shekhar None; Himanshu Shekhar None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 2263. doi:
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      Vishal Raval, Karla P. Mercado-Shekhar, Himanshu Shekhar; Ultrasound Biomarkers: Contrast Enhanced Ultrasound and Nakagami Imaging to Differentiate Benign and Malignant Choroidal Tumor. Invest. Ophthalmol. Vis. Sci. 2024;65(7):2263.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We hypothesized that contrast-enhanced ultrasound (CEUS) using a microbubble technique to quantify microvascular changes and Nakagami imaging for tissue characterization would provide a new approach for diagnosing and differentiating benign and malignant choroidal lesions.

Methods : One patient with choroidal hemangioma (CH) and choroidal melanoma (CM) was selected for CEUS study. Definity®, which contains perflutren microbubbles, was administered as a slow IV bolus (1 ml). CEUS was performed for 1 min post-injection of the dye with video recording. The mean intensity was calculated for each ROI, and the time-averaged difference in pixel intensities of post-injection frames relative to reference pre-injection frame was calculated. Based on the Nakagami statistical distribution model, two Nakagami parameters, m and Ω, where m (shape parameter), representing tissue heterogeneity, and Ω (scale parameter), representing the average energy of backscattered signals, were studied in six patients (CM, 4 patients; CH, 2 patients)

Results : CEUS analysis showed that the time-averaged contrast increased by a percentage difference of 9.9% ± 5.2% for CH and decreased by 4.2% ± 4.7% for CM. Furthermore, the intensity within the normal choroidal region was higher than that within the choroidal tumor region for both CH and CM. Nakagami analysis showed that the m estimates were comparatively higher for hemangioma than for melanoma (7.10 vs 3.73), indicating that hemangioma is a more heterogeneous tumor than melanoma. There was no significant difference in Ω estimates between hemangioma and melanoma (0.65 vs 0.54).

Conclusions : Quantitative intensity-based perfusion analysis using CEUS and backscattering tissue analysis using Nakagami imaging can provide valuable insights to differentiate benign and malignant choroidal lesions.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

 

Figure (A) and (B) Ω estimates of the tumor regions of choroidal melanoma (4 patients) and hemangioma (2 patients). The average of each estimate is shown as a bar.

Figure (A) and (B) Ω estimates of the tumor regions of choroidal melanoma (4 patients) and hemangioma (2 patients). The average of each estimate is shown as a bar.

 

Figure (A) Boxplots of the percentage differences in mean ROI pixel intensities between the reference pre-injection video frame and each of the 50 post-injection frames of the choroidal hemangioma and melanoma. Mean pixel intensities of the normal choroidal region and elevated tumor region for (B) hemangioma and (C) melanoma B-scan data.

Figure (A) Boxplots of the percentage differences in mean ROI pixel intensities between the reference pre-injection video frame and each of the 50 post-injection frames of the choroidal hemangioma and melanoma. Mean pixel intensities of the normal choroidal region and elevated tumor region for (B) hemangioma and (C) melanoma B-scan data.

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