Abstract
Purpose :
This study aimed to investigates the role of Lysine-specific demethylase 5B (KDM5B) in congenital posterior subcapsular cataracts.
Methods :
In this study, we collected four posterior capsular plaque (PCP) and twelve transparent capsule samples. Transcriptome sequencing was applied to identify differential gene expression. We used the Linear Iterative Signature Algorithm (LISA) to predict the transcription factor and calculate each target gene of transcription factor enrichment in the PCP group. Kdm5b loss of function zebrafish was generated using CRISPR/Cas9 technology, and determined through PCR sequencing. Crystalline lens structure and organelle degradation were analyzed and observed through confocal and transmission electron microscopy.
Results :
Transcriptome data from 4 PCP tissues and 12 control samples revealed that most upregulated genes were associated with cytoplasmic ribosomes, angiogenesis, cytokines, cell migration, and extracellular matrix signaling pathways. The LISA algorithm demonstrated an enrichment of KDM5B-regulated genes in the PCP. Ablation of kdm5b induces cataracts in zebrafish models with severe impairment in organelle degradation in lens fiber cells during lens development.
Conclusions :
In this study, we highlighted the regulatory role of KDM5B in the development of congenital posterior subcapsular cataracts.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.