Abstract
Purpose :
In this study, we analyzed the fundus autofluorescence images obtained by wide-field imaging with the help of the image processing software ImageJ, using structures that do not contain endogenous fluorophores, such as the optic disc and vessels. Thus, we aimed to evaluate the age at which fundus autofluorescence can be measured, its age-related changes, and the availability of repeatable measurements.
Methods :
212 eyes of 106 participants aged between 2 and 60, healthy in terms of ophthalmic and systemic diseases, who applied to the ophthalmology clinic of our hospital in November 2023, were included in the study. All images were obtained using a wide-field imaging device, analyzed appropriately with ImageJ software, and then evaluated statistically.
Results :
In the eight macular segments examined in the study, in the four macular quadrants formed by averaging these segments, in three different points determined from the periphery at different distances from the center, and in all the average macular variables calculated by taking the average of the eight quadrants, all showed a statistically significant increase with age in all age groups examined. Superior, inferior and temporal quadrants; Average macular fundus autofluorescence with and without correction is highly, and fundus autofluorescence obtained from the periphery is weakly positively correlated with age. Among the eight macular segments evaluated, the segment with the highest fundus autofluorescence was determined as segment eight, and the quadrant with the highest fundus autofluorescence was determined as the temporal quadrant. Fundus autofluorescence decreases towards the periphery. All evaluated areas show excellent statistical reproducibility, and mean macular measurements with correction have higher agreement values. There is no statistically significant difference between the right and left eye measurements obtained.
Conclusions :
Age and FAF development exhibit a robust correlation evident through wide-field imaging measurements. Repeated measurements show strong statistical consistency. Utilizing vascular and optic disc structures enhances compliance, providing a clearer demonstration of the age-FAF relationship. Wide-field imaging systems, like the one employed here, hold promise for high reproducibility in clinical practice.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.