Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Reinterpretation of the fundus photographic axial retinal vessel reflex
Author Affiliations & Notes
  • Michael Larsen
    Ophthalmology, Rigshospitalet, Copenhagen, Denmark
    Kobenhavns Universitet Det Sundhedsvidenskabelige Fakultet, Kobenhavn, Region Hovedstaden, Denmark
  • Abdullah Amini
    Ophthalmology, Rigshospitalet, Copenhagen, Denmark
    Kobenhavns Universitet Det Sundhedsvidenskabelige Fakultet, Kobenhavn, Region Hovedstaden, Denmark
  • Madeline Evers Olufsen
    Ophthalmology, Rigshospitalet, Copenhagen, Denmark
    Kobenhavns Universitet Det Sundhedsvidenskabelige Fakultet, Kobenhavn, Region Hovedstaden, Denmark
  • Jens F Kiilgaard
    Ophthalmology, Rigshospitalet, Copenhagen, Denmark
    Kobenhavns Universitet Det Sundhedsvidenskabelige Fakultet, Kobenhavn, Region Hovedstaden, Denmark
  • Footnotes
    Commercial Relationships   Michael Larsen None; Abdullah Amini None; Madeline Evers Olufsen None; Jens Kiilgaard None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 6298. doi:
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      Michael Larsen, Abdullah Amini, Madeline Evers Olufsen, Jens F Kiilgaard; Reinterpretation of the fundus photographic axial retinal vessel reflex. Invest. Ophthalmol. Vis. Sci. 2024;65(7):6298.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To identify the origin of the axial retinal vessel reflex seen on fundus photographs and its variation in health and disease.

Methods : Color and infrared fundus photography, OCT, fluorescein angiography in 20 healthy subjects and 20 patients with systemic or local disease and perturbation were examined. Assessment was by visual inspection and grading, validation by review of experimental data from a published study,

Results : The axial reflex was found to increase in intensity with the definition of the patterns of flow on OCT and to be minimal or absent in non-perfused vessels. In eyes with low arterial pressure and poor perfusion, the axial reflex arteries was weak or absent, as was normal intravascular OCT profile of flowing blood. The reflex from the vessel wall was an order of magnitude smaller than that from the flowing blood (Fig. 1).

Conclusions : The axial retinal vessel reflex is predominantly produced by flowing blood, in agreement with erythrocytes being oriented with their flat side facing the vessel wall, flow rates increasing from the vessel wall toward its axis. Hence, the axial reflex will disappear when the movement of blood is arrested. The observations suggest that the enhanced axial reflexes seen in patients with arterial hypertension are caused by vessel narrowing leading to faster flow, to sustain volumetric flow, with increasing velocity gradients (shear rates) leading to an increasingly ordered concentric layering and reflectivity of the erythrocytes.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

 

Infrared fundus photograph (top left) with the upper green line marking the location of an OCT B-scan (top right) that transectsthe retina of a pig with intact circulation, as is evident from the structured figure-of-8 reflex from the circulating blood that fades with increasing scan depth. The vessel to the left is a vein and the one to the right an artery. Shortly after circulatory arrest (bottom left), the retinal vessels can be seen to have contracted and lost most of their axial reflex, the remainder plausibly being attributable to the persistence of the reflex from the anterior vessel wall. The ordered, high-reflectivity signal from inside the vessel has disappeared and been replaced by a weak homogeneous signal (bottom right). Images ccourtesy of ME Olufsen and JF Kiilgaard, https://pubmed.ncbi.nlm.nih.gov/35073557/).

Infrared fundus photograph (top left) with the upper green line marking the location of an OCT B-scan (top right) that transectsthe retina of a pig with intact circulation, as is evident from the structured figure-of-8 reflex from the circulating blood that fades with increasing scan depth. The vessel to the left is a vein and the one to the right an artery. Shortly after circulatory arrest (bottom left), the retinal vessels can be seen to have contracted and lost most of their axial reflex, the remainder plausibly being attributable to the persistence of the reflex from the anterior vessel wall. The ordered, high-reflectivity signal from inside the vessel has disappeared and been replaced by a weak homogeneous signal (bottom right). Images ccourtesy of ME Olufsen and JF Kiilgaard, https://pubmed.ncbi.nlm.nih.gov/35073557/).

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