Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Limitations of Assessing Repeatability of with Coefficient of Variation
Author Affiliations & Notes
  • Elizabeth White
    Ophthalmology, Oregon Health & Science University, Portland, Oregon, United States
  • Dongseok Choi
    Ophthalmology, Oregon Health & Science University, Portland, Oregon, United States
  • Ou Tan
    Ophthalmology, Oregon Health & Science University, Portland, Oregon, United States
  • David Huang
    Ophthalmology, Oregon Health & Science University, Portland, Oregon, United States
  • Yali Jia
    Ophthalmology, Oregon Health & Science University, Portland, Oregon, United States
  • Footnotes
    Commercial Relationships   Elizabeth White None; Dongseok Choi None; Ou Tan None; David Huang Visionix/Optovue, Intalight, Canon, Cylite, Code F (Financial Support), Visionix/Optovue, Genentech, Code P (Patent), Visionix/Optovue, Genentech, Code R (Recipient); Yali Jia Genentech Inc, Code F (Financial Support), Visionix/Optovue Inc, Genentech Inc, Optos , Code P (Patent), Visionix/Optovue Inc, Genentech Inc, Code R (Recipient)
  • Footnotes
    Support  R01 EY023285, R21 EY032146, and P30 EY010572
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 1863. doi:
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    • Get Citation

      Elizabeth White, Dongseok Choi, Ou Tan, David Huang, Yali Jia; Limitations of Assessing Repeatability of with Coefficient of Variation. Invest. Ophthalmol. Vis. Sci. 2024;65(7):1863.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : This study sought to illustrate where within-subject coefficient of variation (wCV) alone may be inconsistent when assessing repeatability and explore an alternative to wCV using ganglion cell complex (GCC) thickness and GCC focal loss volume (GCC-FLV).

Methods : In this abstract, we assess repeatability for GCC thickness and GCC-FLV in normal eyes and eyes with pre-perimetric glaucoma (PPG) or perimetric glaucoma (PG). GCC-FLV integrates fraction loss of GCC in a significant loss mask. Mixed-effects models were used to calculate within-subject standard deviations, wCVs, and ICCs.

Results : thickness (approximately 0.4% for all groups) than for GCC-FLV (>3% for all groups). Additionally, the wCV was more inconsistent across groups for GCC-FLV than for GCC thickness. The wCV for GCC-FLV was over 8.5% for the controls, more than twice that for PG subjects, likely due to the much smaller mean GCC-FLV for controls. In contrast, ICC was consistent across all three groups and for GCC thickness and GCC-FLV (all >0.99), consistently suggesting great repeatability.

Conclusions : wCV was relatively large and inconsistent across groups for GCC-FLV. This was likely misleading as the values for ICC were all nearly 1. For GCC thickness, wCV was very small and consistent across all three groups. This was reflected in the values of ICC also being nearly 1. In conclusion, we recommend using ICC for metrics where the mean value is close to zero in order to assess repeatability as it is consistent than wCV.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

 

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