Abstract
Purpose :
The aim of the study was to check the effect of anti-VEGF agents: bevacizumab, aflibercept, brolucizumab and antioxidants: quercetin, curcumin and resveratrol on the angiogenesis inhibition in the zebrafish larvae laboratory model. The study was focused mainly on observing the development of sub-intestinal venous plexus, the retinal vessels and the phenotype of zebrafish larvae.
Methods :
D. rerio Fli-1a larvae, aged 0 to 5 days post fertilization (dpf) were incubated in the presence of cobalt (II) chloride which promoted pathological angiogenesis. Anti – VEGF and antioxidants were added in different combination and substance concentration of both groups. The angiogenesis inhibition was observed under the microscope in the sub-intestinal venous plexus and the retinal vessels.
Results :
All of the tested anti - VEGF agents compared to cobalt (II) chloride inhibited the angiogenesis and reduced the fluorescence intensity of the examinated areas. Some of antioxidants, when added to anti-VEGF, resulted in more significant angiogenesis inhibition.
Conclusions :
Anti – VEGF agents have an inhibitory effect on angiogenesis in the zebrafish larvae. The addition of antioxidants, in some cases, was helpful to reduce the angiogenesis. The results of the research may contribute to searching for new therapeutic approach in the eye diseases caused by pathological angiogenesis.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.