Abstract
Presentation Description :
Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Our D-FFOCT module design which is coupled to a commercial microscope with a stage top incubator allows non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. We demonstrate long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids, highlighting tissue and cell organization processes such as rosette formation and mitosis as well as cell shape and motility. Monitoring degenerative processes in disease models is faciliated with this live imaging method. Recent developments in D-FFOCT technology are presented, including a self-referenced interferometer design for imaging flat samples.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.