Abstract
Purpose :
We established a system to induce myopia in mice efficiently by putting on a -30D lens (Jiang et al., 2018, Sci Rep), and reported that irradiation of violet light (VL) during the induction prevents from the progression of myopia (Jiang et al., 2021, PNAS). This study aims to determine whether the preventive effect of VL, which had been irradiated before myopia induction, on the progression of myopia persists.
Methods :
Neonatal mice (n=3) were irradiated with VL for 3 weeks (0 to 3 weeks of age), followed by myopia induction using lens-induced myopia (LIM) without VL irradiation. Then, the intraocular refraction and axial lengths (AL) of the eyes were measured using infrared photorefractor and SD-OCT system for mice, respectively. Additionally, a group of 3-week-old mice that did not receive VL irradiation during 0 to 3 weeks of age was also induced to myopia as a control (n=4).
Results :
First, refraction and AL of both VL non-irradiated and irradiated groups of mice were compared after VL irradiation period was finished, and it was confirmed that there were no significantly difference in both refraction (-0.16 ± 1.82 D vs -1.44 ± 3.29 D, p = 0.183) and AL (3.021±0.0122D vs 3.022±0.0282 D, p = 0.436) between VL irradiated and non-irradiated groups.
Next, myopia induction by LIM was performed in right eye of the VL-non-irradiated and VL-irradiated mice, followed by refraction and AL were measured. The intraocular difference of myopic shift was -22.2 ± 4.02 D and -12.8 ± 1.52 D (p = 0.007), in VL-irradiated and non-irradiated groups, respectively. On the other hand, the intraocular difference of AL elongation were +0.0248 ± 0.00411 mm and +0.0137 ±0.00115 mm, in VL-irradiated and non-irradiated groups, respectively (p = 0.064).
Conclusions :
The VL-irradiated mice showed predominant reduction of intraocular refraction and reduction of elongation of axial length compared to controls, although there was no predominant difference in ocular AL elongation. This suggests that the myopia-suppressing effect of VL irradiation is persistent.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.