Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Biofilm formation and its relationship with antimicrobial resistance and clinical outcomes
Author Affiliations & Notes
  • Sujata Das
    Cornea and Anterior Segment, L V PRASAD EYE INSTITUTE, BHUBANESWAR, India
  • Himansu Sekhar Behera
    OCULAR MICROBIOLOGY, L V PRASAD EYE INSTITUTE, BHUBANESWAR, ODISHA, India
  • Akshat Jain
    Cornea and Anterior Segment, L V PRASAD EYE INSTITUTE, BHUBANESWAR, India
  • Smrutirekha Priyadarshini
    Cornea and Anterior Segment, L V PRASAD EYE INSTITUTE, BHUBANESWAR, India
  • Srikant Kumar Sahu
    Cornea and Anterior Segment, L V PRASAD EYE INSTITUTE, BHUBANESWAR, India
  • Footnotes
    Commercial Relationships   Sujata Das None; Himansu Behera None; Akshat Jain None; Smrutirekha Priyadarshini None; Srikant Sahu None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 1108. doi:
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      Sujata Das, Himansu Sekhar Behera, Akshat Jain, Smrutirekha Priyadarshini, Srikant Kumar Sahu; Biofilm formation and its relationship with antimicrobial resistance and clinical outcomes. Invest. Ophthalmol. Vis. Sci. 2024;65(7):1108.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To find the prevalence of biofilm producing bacteria in bacterial keratitis (BK) patients and to compare the resistance pattern and clinical outcome of biofilm producing (BP) and non-biofilm producing bacteria (NBP).

Methods : Bacterial isolates obtained from corneal scrapings were analysed for biofilm
formation with XTT and crystal violet assay in an invitro study. Antimicrobial susceptibility pattern of isolates and clinical outcome were compared.

Results : Of 103 bacterial isolates 75 were found to strong biofilm producer. Resistance pattern of BP and NBP bacteria was found to be; Moxifloxacin (40% in BP vs 33.33% in NBP, p=0.06), Gatifloxacin (41.53% in BP vs 36.36%in NBP, p= 0.06), Ciprofloxacin (35.35% in BP vs 54.54 % in NBP, p=0.39) and Ofloxacin(40 % in BP vs 42.85 % in NBP, p=0.10 ). Out of 75 biofilm producers, 27 (36%) cases had undergone additional surgical procedures.

Conclusions : There is no significant difference in resistance pattern and clinical outcome.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

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