Abstract
Purpose :
To investigate the effects of combination eye drops containing lifitegrast (LF) with anti-inflammatory properties and tocopherol (TO) with antioxidant effects in an experimental dry eye (EDE) mice model
Methods :
EDE was induced in 8-week-old C57BL/6 mice by desiccating stress and injection of scopolamine. The mice were divided into control, EDE, 0.05% cyclosporine (CsA) eye drop, 5% LF eye drop, 10% LF eye drop, and 10% LF+TO eye drop groups. Eye drops 0.05% CsA and 5% LF were administered twice daily, while 10% LF and 10% LF+TO were administered once daily. Tear volume, tear-film break-up time (TBUT), corneal staining scores (CSS), and the tear lipid layer were assessed on days 7 and 14. Additionally, immune T-cell counts and reactive oxygen species (ROS) were evaluated through flow cytometry on day 14.
Results :
Tear volume and TBUT on days 7 and 14 were higher in all treatment groups (0.05% CsA, 5% LF, 10% LF, 10% LF+TO) than in the EDE group. CSS showed a significant decrease in the 10% LF+TO group compared to not only the EDE group but also the 5% LF group after 14 days of treatment. Tear lipid layer scores significantly improved in all treatment groups on day 14 compared to that in the EDE group, and the 10% LF+TO group showed improvement compared to the 5% LF group on days 7 and 14. The conjunctival ROS and CD4+IFN-γ+T cells were significantly decreased in the 10% LF+TO group compared to that in the EDE, 0.05% CsA, 5% LF, and 10% LF groups.
Conclusions :
In the EDE mice model, the 10% LF+TO combination eye drop improved tear volume and ocular surface findings. Additionally, compared to conventional LF eye drops, it demonstrated effectiveness in stabilizing oxidative damage and the tear lipid layer.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.