Abstract
Purpose :
We previously reported the effectiveness of violet light (VL, 360–400 nm wavelength) in suppressing myopia progression in mice by maintaining choroidal thickness through neuropsin-expressing retinal ganglion cells (Jiang X et al., PNAS, 2021). It remains unclear whether the choroidal response to VL occurs locally besides through central innervation. Therefore, we investigated the local control of the choroid.
Methods :
8-week-old C57BL/6J mouse eyes were enucleated. The isolated choroid tissues were embedded in Matrigel matrix and incubated overnight at 37 °C in a CO2 incubator. The tissues were then exposed to 280 µW/cm2 of VL in the range of 360–400 nm for 3 h. At designated time points, the tissues were collected and were immunostained with endomucin for the evaluation of choriocapillaris structures. Stained images of samples were acquired using a confocal laser-scanning microscope and analyzed by AngioTool software.
Results :
The area, junction number, length, and lacunarity of choriocapillaris were compared before and at 3, 7, and 24 h after VL exposure. Compared to before VL exposure, the vessel area (40.7±1.3% vs. 47.2±1.6%, p < 0.05) and junction number (884±92 vs. 1033±50%, p < 0.01) increased significantly 7 h after VL exposure, although there was no significant change in vessel length. The lacunarity, representing gap sizes between the vessels, significantly decreased 7 h after VL exposure (18.3±2.5% vs. 12.9±2.9%, p < 0.05), indicating that VL caused an expansion in the choriocapillaris structure.
Conclusions :
The ex vivo mouse choroid experiment demonstrated local control in the choriocapillaris, indicating vascular structural changes induced by VL.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.