Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Corneal trigeminal nerve -derived extracellular vesicles and their impact on corneal epithelial homeostasis
Deepshikha Tewari; Arnab Roy; Navid Bavi; Khandaker N Anwar; Abhi Krishan; Hamed Massoumi; Tara Nguyen; Mohammad Soleimani; Shen Xiang; Mahbod Baharnoori; Victor H Guaiquil; Mohammad Javad Ashraf; Mark Rosenblatt; Ali R Djalilian; Elmira Jalilian
Author Affiliations & Notes
  • Deepshikha Tewari
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Arnab Roy
    Biochemistry, University of Illinois Urbana-Champaign, Urbana, Illinois, United States
  • Navid Bavi
    Physiology, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Khandaker N Anwar
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Abhi Krishan
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Hamed Massoumi
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
    University of Illinois Chicago Richard and Loan Hill Department of Biomedical Engineering, Chicago, Illinois, United States
  • Tara Nguyen
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Mohammad Soleimani
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Shen Xiang
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Mahbod Baharnoori
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Victor H Guaiquil
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Mohammad Javad Ashraf
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
    Pathology, Shiraj University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
  • Mark Rosenblatt
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Ali R Djalilian
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
  • Elmira Jalilian
    Illinois Eye and Ear Infirmary, University of Illinois, Chicago Department of Ophthalmology and Visual Sciences, Chicago, Illinois, United States
    University of Illinois Chicago Richard and Loan Hill Department of Biomedical Engineering, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Deepshikha Tewari None; Arnab Roy None; Navid Bavi None; Khandaker Anwar None; Abhi Krishan None; Hamed Massoumi None; Tara Nguyen None; Mohammad Soleimani None; Shen Xiang None; Mahbod Baharnoori None; Victor Guaiquil None; Mohammad Ashraf None; Mark Rosenblatt None; Ali Djalilian None; Elmira Jalilian None
  • Footnotes
    Support  National Center for Advancing Translational Sciences, National Institutes of Health, under Grant KL2TR002002, R01 EY024349 (ARD), Core Grant for Vision Research EY01792 (MIR) from NEI/NIH; Research to Prevent Blindness Unrestricted Departmental Grant, Physician-Scientist Award both from Research to Prevent Blindness & UG3/UH3 EY031809 from NEI, NIH Grant P30EY001792, Eversight, and Vision Research Program – Congressionally Directed Medical Research Program VR170180 from the Department of Defense, NIH P30 EY001792, Unrestricted Departmental Grant from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 3690. doi:
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      Deepshikha Tewari, Arnab Roy, Navid Bavi, Khandaker N Anwar, Abhi Krishan, Hamed Massoumi, Tara Nguyen, Mohammad Soleimani, Shen Xiang, Mahbod Baharnoori, Victor H Guaiquil, Mohammad Javad Ashraf, Mark Rosenblatt, Ali R Djalilian, Elmira Jalilian; Corneal trigeminal nerve -derived extracellular vesicles and their impact on corneal epithelial homeostasis. Invest. Ophthalmol. Vis. Sci. 2024;65(7):3690.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The corneal nerves play a crucial role in maintaining the corneal epithelium. This study aims to elucidate the role of extracellular vesicles (EVs) derived from trigeminal nerves (TGs) in corneal epithelial homeostasis (CoEpiH), a critical aspect of corneal health and visual acuity. It focuses on the interplay between TG-derived EVs and corneal epithelial cells (CoEpiCs), highlighting their potential in regulating corneal functions and integrity.

Methods : TGs were isolated from C57BL/6 mice and cultured in vitro. Their neuronal identity was confirmed via immunostaining and flow cytometry using Tuj1. TG-derived EVs were isolated through multiple centrifugation steps and characterized using Cryo-Electron Microscopy (Cryo-EM), Nanosight, ExoView, and Western blot analysis. The effects of TG-derived EVs on CoEpiCs were assessed in vitro, examining cell proliferation and migration. Additionally, the impact on corneal epithelial wound healing was evaluated both in vitro and in vivo via subconjunctival injection in nonpathological conditions, followed by immunostaining and slit-lamp imaging.

Results : Cryo-EM confirmed the vesicular morphology of TG-derived EVs. Nanosight analysis indicated a higher abundance of EVs in TGs compared to controls. ExoView revealed the expression of tetraspanin markers (CD81, CD63, CD9) on EVs, with a notable predominance of CD81. Western blot analysis supported these findings. In vitro studies demonstrated a significant increase in CoEpiC proliferation and migration (P<0.05) with the uptake of TG EVs by approximately 45% of CoEpiCs. Moreover, both in vitro and in vivo studies showed that TG EVs significantly enhanced corneal epithelial wound healing, with notable wound closure observed after 28 hours (p<0.05) compared to controls.

Conclusions : This study highlights the crucial role of neuronal-derived EVs in maintaining ocular surface homeostasis and suggests the potential for developing novel therapeutics targeting EV-mediated pathways in corneal and other ocular diseases.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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