Abstract
Purpose :
The lymphatic system plays a critical role in tissue fluid regulation and immune response. Lymphedema is a common pathological condition in which tissue fluid accumulates due to lymphatic dysfunction. To date, there is still a lack of experimental models and therapeutic approaches for this disorder. The conjunctiva is endowed with an extensive lymphatic network. It offers an ideal site to study lymphedema due to its accessible location and transparent nature. In this study, we developed a novel lymphedema model in the conjunctiva and presented its advantages to study the process of lymphedema in vivo and in real time.
Methods :
Fluorescent reporter mice of Prox-1, the master control gene for lymphatic development, were used in this study. Conjunctival lymphatics were visualized under an advanced live imaging system. A lymphatic incision was made at the trunk of the superior collecting lymphatics. For sham procedure, the incision was made close to but avoiding the collecting lymphatics. The conjunctiva was examined by ophthalmic slit-lamp microscopy, optical coherence tomography (OCT), and intravital microscopy. Moreover, functional analysis was performed by subconjunctival dye injection.
Results :
Conjunctival edema was observed after the lymphatic incision procedure. Compared to the control condition, increased tissue thickness was detected by OCT. Lymphatic dilation and fluid drainage obstruction were detected by the advanced live imaging system.
Conclusions :
In this study, we have successfully established a novel model to study lymphedema in the conjunctiva. Future studies using this model may provide new insights into the pathogenesis and management of this devastating disease that occurs both inside and outside the eye.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.