Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Investigation of YAP signaling Expression in Keratoconic eyes.
Author Affiliations & Notes
  • Yohei Yamashita
    Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
  • Koji Kitazawa
    Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
  • Haruka Yanagisawa
    Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
  • Yasufumi Tomioka
    Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
  • Chie Sotozono
    Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
  • Footnotes
    Commercial Relationships   Yohei Yamashita None; Koji Kitazawa None; Haruka Yanagisawa None; Yasufumi Tomioka None; Chie Sotozono Santen, Code F (Financial Support), CorneaGen, Code F (Financial Support), Sancontact Lens, Code F (Financial Support)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 4556. doi:
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      Yohei Yamashita, Koji Kitazawa, Haruka Yanagisawa, Yasufumi Tomioka, Chie Sotozono; Investigation of YAP signaling Expression in Keratoconic eyes.. Invest. Ophthalmol. Vis. Sci. 2024;65(7):4556.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Keratoconus is a condition where the cornea progressively bulges and deforms. There is growing evidence that the Hippo-YAP/TAZ signaling pathway controls and regulates the three-dimensional architecture of organs. This study aims to investigate the expression of YAP and cyclin-dependent kinase7 (CDK7), which is supposed to be an upstream regulator of YAP/TAZ, in keratoconus by means of immunostaining.

Methods : The study targeted cases that underwent penetrating keratoplasty for keratoconus, with the donors serving as normal ocular controls. Immunostaining was performed to detect the presence of YAP, CDK7, and factors involved in corneal healing and fibrosis, such as SMAD3 and SMAD7.

Results : Three cases of corneal tissue from keratoconus and three cases of normal donor corneal tissue as controls were immunostained. Immunostaining of all three keratoconus corneas was positive for YAP, CDK7, SMAD3 and SMAD7, and their expression levels were elevated compared to normal corneas. The expression of YAP, CDK7, SMAD3 and SMAD7 was also observed in the corneal stroma as well as the corneal epithelium, while, the normal corneas were negative for all stains.

Conclusions : The presence of YAP signaling in the keratoconic corneas indicates its involvement in the pathology of keratoconus, suggesting a potential role for YAP in this condition.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

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