Abstract
Presentation Description :
Studies in vitro have shown that activated macrophages undergo profound metabolic reprogramming. The tools to study the intracellular metabolism of immune cells within a native microenvironment in a live animal are limited. In this study we explored the use of autofluorescence lifetime imaging microscopy (FLIM) of NAD(P)H and FAD using various tail fin injury models of larval zebrafish that each induce a characteristic macrophage inflammatory response. We found that FLIM is capable of detecting differences between the intracellular metabolism of pro-inflammatory and pro-healing macrophage populations at the wound microenvironment. These metabolic differences correlated with the wound healing capacity of the larvae. We are continuing to develop and apply FLIM for metabolic imaging in vivo, so this technology can be leveraged in the field of immunometabolism.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.