Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Follow up Analysis in Patients with Hydroxychloroquine Toxicity Using Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO)
Author Affiliations & Notes
  • Coby Soule
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Taylor Joseph Johnson
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Alexandra Vitale
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Marcela Pasaye
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Lydia Sauer
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Spencer Fuller
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Paul S Bernstein
    University of Utah Health John A Moran Eye Center, Salt Lake City, Utah, United States
  • Footnotes
    Commercial Relationships   Coby Soule None; Taylor Johnson None; Alexandra Vitale None; Marcela Pasaye None; Lydia Sauer None; Spencer Fuller None; Paul Bernstein Heidelberg Engineering, Code F (Financial Support)
  • Footnotes
    Support  VitreoRetinal Surgery Foundation
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 5896. doi:
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      Coby Soule, Taylor Joseph Johnson, Alexandra Vitale, Marcela Pasaye, Lydia Sauer, Spencer Fuller, Paul S Bernstein; Follow up Analysis in Patients with Hydroxychloroquine Toxicity Using Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). Invest. Ophthalmol. Vis. Sci. 2024;65(7):5896.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Hydroxychloroquine (HCQ) is a drug commonly used to treat a variety of autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus. A side-effect of the medication is development of irreversible retinal damage, which progresses up to 6 months after stopping HCQ. Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) has been shown to highlight retinal changes related to HCQ toxicity. The present analysis aims to follow up on our group’s baseline study to characterize the longitudinal FLIO changes related to HCQ use in order to determine if FLIO is an effective modality for ongoing monitoring.

Methods : 28 eyes of 14 patients were investigated in this longitudinal study with a mean follow-up time of 4 years + 11 months. Clinical grading of HCQ toxicity was confirmed by retinal specialists (PSB, SDF). FLIO images of a 30° retinal field were acquired in short (498-560 nm, SSC) and long (560-720 nm, LSC) spectral wavelength channels using the same first-generation prototype Heidelberg Engineering Spectralis-based FLIO. Mean autofluorescence lifetimes (tm) were calculated and compared between baseline and follow-up visits.

Results : Of the 4 eyes that had toxicity at baseline from patients who had stopped HCQ prior to their initial encounter, there was no significant prolongation of FLIO lifetimes in the area of toxicity (SSC: p=0.264, LSC: p=0.721). Of the 24 eyes that did not have toxicity at baseline from patients who continued to take HCQ, a significant prolongation was found in the SSC (243 ± 49 ps at baseline versus 272 ± 39 ps at follow-up, p<0.01). There was no significant prolongation in the LSC (302 ± 50 ps at baseline versus 314 ± 52 ps at follow-up). 2 eyes were excluded due to development of cataract.

Conclusions : Patients without HCQ toxicity who continued to take HCQ showed prolonged FLIO lifetimes 4-5 five years after initial imaging. Patients with prior toxicity who did not take HCQ did not show prolongation of FLIO lifetimes. These results suggest that FLIO is a useful tool to monitor the progression of HCQ-related retinal toxicity. We aim to follow up on all patients initially included in our study to further understand the progression of retinal changes.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

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