Abstract
Purpose :
We report a Tyr-759 (F-759) mutation mouse, a new Meibomian gland (MG) inflammatory animal model of Meibomian gland dysfunction (MGD).
Methods :
Rheumatoid arthritis (RA) model animal, F-759 mutation mouse known as IL-6 over-expression model mouse and Meibomian gland dysfunction (MG plugging score, lid margin irregularity score) of wild type mouse (C57BL/6), Corneal fluorescein (CF) and histological examination (H & E staining, Oil Red O staining) were performed, and differences between the two mice were compared.
Results :
Upper lid and lower lid MG plugging scores were significantly higher in F-759 mutation mice. Also, the CF of F-759 mice was significantly higher than that of the wild type. As a result of histological examination, Meibomian gland dilatation was observed more prominently in F-759 mutation mice than in wild type mice in H & E staining, and lipid droplets were observed less in F-759 mutation mice than in wild type in Oil Red O staining.
Conclusions :
In F-759 mutation mice, IL-6 over-expression and MGD are very closely related, and F-759 mutation mice are thought to be a new model to study the pathophysiology of MGD.
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.