Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
Abnormal anatomy and function in the aged mouse retina
Author Affiliations & Notes
  • Priyamvada M Pitale
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Solomon Gibson
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Caroline Keehn
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Arman T Yazdian
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Guofu Shen
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Benjamin J Frankfort
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Priyamvada Pitale None; Solomon Gibson None; Caroline Keehn None; Arman Yazdian None; Guofu Shen None; Benjamin Frankfort None
  • Footnotes
    Support  NIH Grants EY025601 and EY002520; Research to Prevent Blindness Unrestricted Award
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 6757. doi:
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      Priyamvada M Pitale, Solomon Gibson, Caroline Keehn, Arman T Yazdian, Guofu Shen, Benjamin J Frankfort; Abnormal anatomy and function in the aged mouse retina. Invest. Ophthalmol. Vis. Sci. 2024;65(7):6757.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have previously shown that inner retinal capillary plexi (RCPs) and retinal ganglion cells (RGCs) display age-related phenotypes. In this project, we aim to better understand the anatomic and physiologic deficits that occur in similarly aged mice as a part of normal aging.

Methods : All experiments were conducted using aged (85 weeks) c57BL/6J mice while young (16 weeks) mice served as controls. We used ocular coherence tomography (OCT) and H&E staining to assess gross retinal anatomy. In another set of retinas, we used transmission electron microscopy (TEM) to analyze retinal microvasculature. Lastly, we performed electroretinograms (ERGs) to analyze retinal physiology.

Results : OCT analysis showed that aged retinas have reduced inner retinal (retinal nerve fiber layer, inner plexiform layer and inner nuclear layer) and overall retinal thickness (*p≤0.05) compared to young retina controls. TEM analysis of aged retinas showed prominent dilatation of the vascular basement membrane in the superficial (***p≤0.001), intermediate (****p≤0.0001), and deep (***p≤0.001) RCP compared to young retinas. ERG assessment showed decreased amplitudes in the a-wave (***p≤0.001), b-wave (****p≤0.0001), and pSTR (***p≤0.001) which correspond to photoreceptor, bipolar cell, and RGC activity, respectively. Analysis of H&E sections is ongoing.

Conclusions : These data suggest that, in addition to the previously described microvasculature changes seen in the RCPs, there may be additional disruptions to capillary basement membranes and neuronal function in the aged retina.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

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