Investigative Ophthalmology & Visual Science Cover Image for Volume 65, Issue 7
June 2024
Volume 65, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2024
A randomized, active-controlled, patient and investigator-masked, multiple dose Ph2 study of intravitreal LKA651, an anti-erythropoietin (EPO) antibody fragment, in patients with diabetic macular edema
Author Affiliations & Notes
  • Nadia Zakaria
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Danielle Spera
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Katy Hayes
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Tingting Zhai
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Brijesh Yadav
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Jorge Aranda
    Ophthalmology Research, Novartis AG, Cambridge, Massachusetts, United States
  • Darlene Lu
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Melissa Liew
    Translational Medicine, Novartis AG, Cambridge, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Nadia Zakaria Novartis, Code E (Employment); Danielle Spera Novartis, Code E (Employment); Katy Hayes Novartis, Code E (Employment); Tingting Zhai Novartis, Code E (Employment); Brijesh Yadav Novartis, Code E (Employment); Jorge Aranda Novartis, Code E (Employment); Darlene Lu Novartis, Code E (Employment); Melissa Liew Novartis, Code E (Employment)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2024, Vol.65, 6262. doi:
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      Nadia Zakaria, Danielle Spera, Katy Hayes, Tingting Zhai, Brijesh Yadav, Jorge Aranda, Darlene Lu, Melissa Liew; A randomized, active-controlled, patient and investigator-masked, multiple dose Ph2 study of intravitreal LKA651, an anti-erythropoietin (EPO) antibody fragment, in patients with diabetic macular edema. Invest. Ophthalmol. Vis. Sci. 2024;65(7):6262.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Intraocular EPO concentrations have been shown to be elevated in eyes with DME (Lim and Han, 2011), and in preclinical studies, EPO over-expression in mouse models contributes to retinal vascular pathologies [IOVS 60(9):3664]. The purpose of this study was to evaluate the safety and efficacy of IVT LKA651 (anti-EPO Fab), administered as monotherapy or in combination with Lucentis, in patients with diabetic macular edema (DME).

Methods : This study was a 3-arm, parallel group, randomized, patient- and investigator-masked trial planned in 90 patients with DME. Patients were enrolled into 1 of 3 arms: LKA651 (5mg) monotherapy, LKA651 (1mg) plus Lucentis combotherapy, and Lucentis monotherapy. Patients were dosed 3 times in 4-week intervals in the treatment phase and then followed up for an additional 12 weeks in a treatment free extension.

Results : The baseline demographic and disease characteristics were generally well balanced between the treatment arms. Of the 91 treated patients, majority were male (64.8%), White (90.1%), and the mean (± SD) age was 62.2 (± 8.60) years. Positive safety and tolerability profile of LKA monotherapy and combotherapy, was observed. There was no significant difference in the incidence of patients with severe AEs amongst the treatment arms. Both LKA monotherapy and combotherapy arms showed BCVA gains from baseline. A 4.9 letter mean improvement was observed from baseline in the LKA arm, which was lower than the 7.6 letters observed in the Lucentis arm and 9.5 letters observed in the combotherapy arm. The difference in MMRM model based mean estimates (90% CI; p-value) in BCVA at Week 12 was –2.7 (−6.6, 1.3; 0.866) letters for LKA monotherapy vs Lucentis, and +2.0 (−1.9, 5.8; 0.198) letters for LKA/Lucentis combotherapy vs Lucentis. LKA combotherapy demonstrated reduction in CSFT from baseline but this was not superior to reduction observed in the Lucentis comparator arm.

Conclusions : Anti-EPO is a novel mechanistic approach to the treatment of DME. Positive safety and tolerability was observed for IVT doses of LKA651 monotherapy or LKA651 dosed in combination with Lucentis® in patients with DME. Treatment with IVT LKA651 showed clinically meaningful efficacy data with improvement in BCVA and CSFT from baseline and a positive safety and tolerability profile.

This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.

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