Abstract
Purpose :
This study investigates the influence of intermittent fasting (IF) on gut microbiota composition and intestinal metabolomics in a murine model of diabetes
Methods :
A C57BL/6J mouse model was employed, comprising a total of 20 mice. The experimental groups included 5 mice each for the normal control (NC), diabetic (DM), diabetic intermittent fasting (DF), and normal intermittent fasting (NF) groups. Intermittent fasting was implemented at 24-hour intervals, and the experiment spanned six months. Following the intervention, fecal samples were collected for 16S rRNA sequencing and fecal metabolomics analysis
Results :
Intermittent fasting significantly altered the composition of the gut microbiota. In comparison to the NC group, the NF group exhibited a significant increase in both alpha and beta diversity, while diversity in the DF group further decreased compared to the DM group. The relative abundance was also notably altered in the IF intervention groups, with a decrease observed in key genera such as Clostridium_XlVa, Desulfovibrio, Acetatifactor, and Oscillibacter. Metabolomic analysis revealed significant differences among the four experimental groups
Conclusions :
Intermittent fasting induces significant changes in gut microbiota composition and metabolomic profiles in a murine model of diabetes. The observed alterations in key microbial taxa and metabolites suggest potential therapeutic implications for managing diabetes through dietary interventions. These findings contribute to our understanding of the intricate relationship between intermittent fasting, gut microbiota, and diabetes
This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024.