RNA from cultured BV-2 microglia cells was isolated with the RNA Isolation Kit following the manufacturer's instructions (Machery & Nagel, Düren, Germany). RNA from retinas was isolated with the Qiagen Micro isolation kit according to the manufacturer’s protocol. Purity and integrity of the RNA was assessed with a NANODrop 2000 machine (Thermo Fisher Scientific, Waltham, MA, USA). The cDNA was synthesized with the Thermo Fischer Reverse Transcriptase Kit according to the company's protocol (Thermo Fisher Scientific, Waltham, MA, USA). Subsequent quantitative real-time PCR (qRT-PCR) analysis was performed in duplicates with either the Takyon Probe Assay protocol (Eurogentec Deutschland GmbH, Cologne, Germany) or Takyon SYBR Green Assay protocol using the LightCycler 480 II (Roche, Basel, Switzerland). Primer sequences and Roche Universal Probe Library probe numbers for probe-based assay were as follows ATP synthase, H+-transporting, mitochondrial F1 complex, β polypeptide (Atp5b), forward primer 5′-ggcacaatgcaggaaagg-3′, reverse primer 5′-tcagcaggcacatagatagcc-3′, probe #77; Complement C1q A chain (C1qa), forward primer 5′-ggagcatccagtttgatcg-3′, reverse primer 5′-catccctgagaggtctccat-3′, probe #16; Complement component 3 (C3), forward primer 5′-accttacctcggcaagtttct-3′, reverse primer 5′-ttgtagagctgctggtcagg-3′, probe #76; complement component 6 (C6), forward primer 5′-AAGGAAGACACGTGCACCAA-3′, reverse primer 5′- TCCTTCACCGATTCTAGCCAC -3; complement component 7 (C7), forward primer 5′-GGTGTGCTTTATAGCAGCGTT-3′, reverse primer 5′-CTGAACGCCTTCGAGTCTGAG-3; complement component 8a (C8a), forward primer 5′-AAACGCCACCTGGTGTGTAA-3′, reverse primer 5′-AGGATGTTGTACCCCAAGGC-3; complement component 9 (C9), forward primer 5′-CAGCAGGCTATGGGATCAACA-3′, reverse primer 5′-CGGTCACAGAGTCCGTTGTA-3; complement factor b (Cfb) forward primer 5′-ctcgaacctgcagatccac-3′, reverse primer 5′-tcaaagtcctgcggtcgt-3′, probe #1; complement factor h (Cfh), forward primer 5′-gaaaaaccaaagtgccgaga-3′, reverse primer 5′-ggaggtgatgtctccattgtc-3′, probe #25; inducible nitric oxide synthase (iNos), forward primer 5′-ctttgccacggacgagac-3′, reverse primer 5′-tcattgtactctgagggctga-3′, probe #13; Translocator protein (Tspo), forward primer 5′-cccttgggtctctacactgg-3′, reverse primer 5′-aagcagaagatcggccaag-3′, probe #21. ATPase was used as reference gene and the ΔΔC method was applied using the LightCycler 480 software 1.2.1 for data evaluation.