Investigative Ophthalmology & Visual Science Cover Image for Volume 66, Issue 3
March 2025
Volume 66, Issue 3
Open Access
Retinal Cell Biology  |   March 2025
Dicer Loss in Müller Glia Leads to a Defined Sequence of Pathological Events Beginning With Cone Dysfunction
Author Affiliations & Notes
  • Daniel Larbi
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Alexander M. Rief
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Seoyoung Kang
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Shaoheng Chen
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Khulan Batsuuri
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Sabine Fuhrmann
    Ophthalmology and Visual Sciences Department, Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee, United States
  • Suresh Viswanathan
    Indiana University School of Optometry, Bloomington, Indiana, United States
  • Stefanie G. Wohl
    Department of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States
  • Correspondence: Stefanie G. Wohl, Department of Biological and Vision Sciences, The State University of New York College of Optometry, 33 West 42nd Street, New York, NY 10036, USA; [email protected]
Investigative Ophthalmology & Visual Science March 2025, Vol.66, 7. doi:https://doi.org/10.1167/iovs.66.3.7
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      Daniel Larbi, Alexander M. Rief, Seoyoung Kang, Shaoheng Chen, Khulan Batsuuri, Sabine Fuhrmann, Suresh Viswanathan, Stefanie G. Wohl; Dicer Loss in Müller Glia Leads to a Defined Sequence of Pathological Events Beginning With Cone Dysfunction. Invest. Ophthalmol. Vis. Sci. 2025;66(3):7. https://doi.org/10.1167/iovs.66.3.7.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The loss of Dicer in Müller glia (MG) results in severe photoreceptor degeneration, as it occurs in retinitis pigmentosa or age-related macular degeneration; however, the sequence of events leading to this severe degenerative state is unknown. The aim of this study was to conduct a chronological functional and structural characterization of the pathological events in MG-specific Dicer-conditional knockout (cKO) mice in vivo and histologically.

Methods: To delete Dicer and mature microRNAs (miRNAs) in MG, two conditional Dicer1 knockout mouse strains (Rlbp-CreER:tdTomato:Dicer-cKOMG and Glast-CreER:tdTomato:Dicer-cKOMG) were created. Optical coherence tomography (OCT), electroretinograms (ERGs), and histological analyses were conducted to investigate structural and functional changes up to 6 months after Dicer deletion.

Results: Dicer/miRNA loss in MG leads to (1) impairments of the area spanning from the external limiting membrane (ELM) to the retinal pigment epithelium (RPE), (2) cone photoreceptor dysfunction, and (3) retinal remodeling and functional loss of the inner retina at 1, 3, and 6 months after Dicer loss, respectively, in both of the knockout mouse strains. Furthermore, in the Rlbp-CreER:tdTomato:Dicer-cKOMG strain, rod photoreceptor impairment was found 4 months after Dicer depletion (4) accompanied by alteration of RPE integrity (5).

Conclusions: MG Dicer loss in the adult mouse retina impacts cone function prior to any measurable changes in rod function, suggesting a pivotal role for MG Dicer and miRNAs in supporting cone health. A partially impaired RPE, however, seems to accelerate rod degeneration and overall degenerative events.

Retinal degenerative diseases, including age-related macular degeneration (AMD) or retinitis pigmentosa (RP), cause vision impairment and eventually lead to blindness. Although many retinal dystrophies can be linked to mutations in specific genes, non-genetic/epigenetic factors also contribute to disease progression.16 MicroRNAs (miRNAs) belong to non-coding RNAs and act as epigenetic factors by repressing translation. They play a role in retinal disease progression and very likely in disease onset and manifestation, as some of them are cell type–specific (see review Zuzic et al.2). miRNAs require processing before they obtain their mature functional form, which occurs via special enzyme complexes, including the Dicer complex. Hence, deleting Dicer1 in retinal cells (photoreceptors or MG) or the RPE is a very efficient way to study the role of cell type–specific mature miRNAs.710 
MG are the primary glia in the retina and fulfill a variety of functions in the healthy retina, including but not limited to (1) feeding neurons with lactate, (2) neurotransmitter recycling (via glutamine synthetase), (3) water ion homeostasis (via water pores and ion pumps), (4) structural stability and integrity via their long processes that span the entire tissue and form the internal/inner limiting membrane (ILM) toward the vitreous and external or outer limiting membrane (ELM/OLM) toward the RPE, (5) cone photopigment recycling, and (6) formation of the blood–retinal barrier (BRB) by wrapping blood vessels.1118 Because MG have such myriad functions, it is not far fetched to assume that MG impairment would result in devastating outcomes. Indeed, partial elimination of MG via toxins leads to activation of the remaining glia, disruption of the BRB, and photoreceptor loss resembling degenerative diseases such as AMD, diabetic retinopathy, or macular telangiectasia 2 (MacTel2).19,20 This suggests that MG are not only responders to damage but also potential initiators of retinal degeneration/retinal dystrophies. 
We previously established an MG-specific Dicer knockout mouse and showed that the loss of mature miRNAs in the glia leads to a slow-progressing phenotype that resembles retinitis pigmentosa several months after the manipulation.9 Intriguingly, MG miRNAs were reduced by about 80% as early as 1 month after Dicer deletion.9,21 The overall retinal histology, however, appeared unaltered at the time. Only occasionally, the tissue seemed to have some enlarged areas (bulgy appearance), but whether a postmortem event caused this outcome was unclear. Furthermore, even 3 months after Dicer loss, the overall retina was histologically intact, with only occasional alterations at the cellular level (MG displacement). Later, though, several substantial alterations were found, including a thin outer nuclear layer (ONL). How this pathophysiological phenotype developed, when it manifested, and whether the RPE contributed to the outcome were, however, not investigated and are not known. Therefore, the aims of this study were (1) to investigate the impact of the loss of Dicer in adult MG with regard to onset, manifestation, and progression of pathophysiological events in the retina; and to (2) elaborate the contribution of the RPE to the pathophysiological phenotype. 
We used two MG-specific Dicer knockout strains—the aforementioned Rlbp-CreER:tdTomato:Dicer-cKOMG mouse9 and a newly generated Glast-CreER:tdTomato:Dicer-cKOMG—and conducted spectral-domain optical coherence tomography (SD-OCT) and electroretinography (ERG) coupled with histological analysis. We analyzed the mice at early (1 month after Dicer deletion), intermediate (3–4 months after Dicer deletion), and late stages (6 months after Dicer deletion). We found that the first dysfunction concerned cone photoreceptors, followed by rod loss and functional loss of inner retinal neurons at a late stage. The degenerative events were more accelerated in the Rlbp-CreER:tdTomato:Dicer-cKOMG mouse that also showed partial RPE impairments. 
Methods
Animals and Cre Induction
All mice used in this study were housed at the State University of New York, College of Optometry, in accordance with the Institutional Animal Care and Use Committee–approved protocols and ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. The Rlbp1-CreERT2 strain was obtained from Edward Levine, PhD, Vanderbilt University.22 The Glast-CreER strain (Tg[Slc1a3-cre/ERT]1Nat, ID 012586, generated by Jeremy Nathans, MD, PhD, Johns Hopkins University), the R26-stop-flox-CAG-tdTomato strain (Ai14, #007908), and the Dicer conditional knockout strain (Dicerf/f, #006001, generated by Brian Harfe, PhD,23) were obtained from The Jackson Laboratories (Bar Harbor, ME, USA) to create the following wildtype and Dicer-conditional knockout (cKO) strains: (1) Rlbp1-CreERT2: stopf/f-tdTomato (referred to as Rlbp-Cre:tdTomato or wildtype) and corresponding (2) Rlbp1-CreERT2: stopf/f-tdTomato: Dicerf/f (referred to as Rlbp-Cre:tdTomato:Dicer-cKOMG, or abbreviated Rlbp-Cre:Dicer-cKOMG), as well as (3) Glast1-CreER: stopf/f-tdTomato (referred to as Glast-Cre: tdTomato or wildtype) and corresponding (4) Glast1-CreER: stopf/f-tdTomato: Dicerf/f (referred to as Glast-Cre:tdTomato:Dicer-cKOMG, or abbreviated Glast-Cre:Dicer-cKOMG). Males and females were used. Genotyping was done using the primers listed in Supplementary Table S1. Tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) was administered intraperitoneally at 75 mg/kg in corn oil for 4 consecutive days, for Dicer deletion at P11–14, to initiate the recombination of the floxed alleles. Furthermore, S129 mice (Rlbp1-CreER background strain), tdTomato mice (C57Bl6 background strain), and Cre-negative mice were used as controls and were compared to tamoxifen-treated wildtype mice to exclude the possibility of a treatment effect or any abnormality of the wildtype controls used. 
Retinal SD-OCT Imaging
In vivo retinal structure was assessed using the Envisu R2200 SD-OCT device (Bioptigen, Durham, NC, USA). Animals were anesthetized via intraperitoneal injection of 75 to 100 mg/kg ketamine and 5 to 10 mg/kg xylazine dissolved in sterile saline. Pupils were dilated with phenylephrine hydrochloride (2.5%) and tropicamide (0.5%). Corneas were kept lubricated during scans using methylcellulose. Rectangular and radial volume scans (1.4 × 1.4 mm, 1000 A-scans/B-scans × 15 frames/B-scan) were obtained while centered on the optic nerve head. For image analysis, retinal layers were manually segmented in a 9 × 9 plot in four regional quadrants (superior–inferior, nasal–temporal), and thicknesses were measured using the Diver 2.4 software. Total retinal thickness was defined as the distance from the inner border of the retinal nerve fiber layer (NFL) and the outer border of the retinal pigment epithelium (RPE). All nuclear and plexiform layers were analyzed including the NFL/ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer, and ONL. The region containing the inner and outer segments of photoreceptors and the RPE could not be distinguished with high resolution. Therefore, the distance between the outer border of the ELM (also known as the outer limiting membrane [OLM]) to the outer border of the RPE was consolidated as ELM–RPE area as described previously.24,25 Thickness measurements were conducted in the central areas, ∼650-µm radius from the optic nerve (ON). To create a timeline of thickness changes in different retinal layers, we averaged the thicknesses measured at various retinal eccentricities in both the superior–interior and nasal–temporal directions for each time point of analysis. 
Electroretinogram Recordings
Mice were dark adapted overnight and anesthetized via an intraperitoneal injection of 75 to 100 mg/kg ketamine and 5 to 10 mg/kg xylazine dissolved in sterile saline. Recordings were performed in the dark room using the Espion Electrophysiology System (Diagnosys LLC, Lowell, MA, USA). Pupils were dilated with 1% tropicamide ophthalmic solution, and the eyes were lubricated using 1% methylcellulose. Animals were kept on a heat pad during the recording to maintain constant body temperature. The Espion Electrophysiology System uses a gold wire electrode as a recording electrode, with needles inserted into the cheek and back as reference and ground electrodes, respectively; a hand-held portable stimulator is the light source. Fullfield 5-ms flashes were used to elicit responses. ERGs were obtained with light intensities ranging from 0.001 to 64 cd·s/m2 (scotopic) and 0.06 to 17.5 cd·s/m2 (photopic). Scotopic and photopic ERG a-wave amplitudes were measured from the baseline of the waveform to the trough of the initial negative deflection, and the b-wave amplitudes were measured from the trough of the initial negative deflection to the peak of the subsequent positive deflection. For an in-depth analysis of the scotopic b-wave, the Naka–Rushton equation was used to fit the intensity–response amplitudes:  
\begin{eqnarray}V\left( I \right) = {{V}_{max}}\left( {{{I}^n}} \right)/\left( {{{I}^n} + {{K}^n}} \right)\end{eqnarray}
(1)
where I is the stimulus intensity; V is the amplitude at stimulus intensity I; Vmax is the maximum/saturated amplitude and an indirect measurement for responsiveness; K is the semi-saturation constant and represents the stimulus intensity I at which half of the saturated amplitude Vmax is reached and hence is an indirect measurement for sensitivity; and n is the slope of the fit curve and an indirect measurement of gain/heterogeneity.26,27 
Tissue Preparation
Mice were euthanized and eyes were marked at the nasal side, enucleated, and transferred to prechilled phosphate-buffered saline (PBS) for eye cup preparation or Hank's Balanced Salt Solution (HBSS) for RPE preparation. 
Retinal Cross-Sections
The eyeball was prefixed in 4% paraformaldehyde (PFA) at 4°C for 20 minutes. After removal of the cornea, lens, iris, and vitreous body in PBS, the eyecups were fixed in cold 4% PFA for an additional 20 minutes, washed for 10 minutes in prechilled PBS, and incubated with 30% sucrose in PBS overnight at 4°C. The tissue was embedded with a nasal–temporal orientation in optimum cutting temperature (OCT) embedding medium and frozen at –80°C. The frozen tissue was cross-sectioned in 12 µm thick sections for subsequent immunofluorescent labeling. 
Retinal Flatmounts
The unfixed eyeball was carefully dissected in cold PBS to remove the cornea, lens, iris, ciliary body, and vitreous body. The retina was carefully lifted from the RPE and transferred to a slide. To flatten the retina, four symmetric radial cuts were made. The tissue was subsequently fixed with cold 4% PFA for 20 minutes at room temperature (RT), washed three times for 10 minutes in PBS, and stained. 
RPE Flatmounts
RPE preparation was conducted as described before.28 In brief, muscles and connective tissue on the sclera were removed to facilitate the tissue flattening. Cornea, iris, lens, and vitreous body were removed, and the RPE (with attached retina) was carefully transferred on filter paper. Tissue was cut radially, and the retina was gently separated. The RPE (with sclera) was fixed with cold 4% PFA for up to 2 hours at RT, washed three times in PBS at RT for 10 minutes, and stained. 
Immunofluorescent Labeling
For immunofluorescent staining, frozen sections were dried at 37°C for 20 minutes, fixed for 20 minutes with 4% PFA, and washed in PBS. Sections or flatmounts were incubated in blocking solution (5% milk block comprised of 2.5 g nonfat milk powder in 50 mL PBS, with 0.5% Triton X-100 or 5% horse serum with 0.5% Triton X-100) for at least 1 hour at RT. After blocking, the tissue was incubated with primary antibodies (Supplementary Table S2) in 5% milk block or horse serum overnight, and retinal flatmounts were incubated for 2 to 3 days. After three thorough washes in PBS, the tissue was incubated with secondary antibodies (1:500–1000; Supplementary Table S2) for 1 hour (slides) or 1 day (flatmounts) at RT and then counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 1:1,000; Sigma-Aldrich). After incubation, the tissue was washed three times in PBS and mounted with cover glasses and mounting medium (Invitrogen). 
Fluorescence Microscopy, Confocal Laser Scanning Microscopy, and Image Processing
For whole tissue imaging, retinal eye cups were imaged using a fluorescent microscope (Keyence, Indianapolis, IN, USA) equipped with a 4× objective. Immunofluorescent-labeled retinal cross-sections, RPE flatmounts, and retinal flatmounts were imaged using a confocal laser scanning microscope (Fluoview FV1200; Olympus, Tokyo, Japan) equipped with 4×, 10×, 20×, or 40× objectives and Fluoview FV31S software. Images were processed and analyzed using Adobe Bridge and Photoshop (Adobe, San Jose, CA, USA) and Affinity software (West Bridgford, UK). 
Statistical Analysis
Statistical analyses were performed using the Mann–Whitney U test. The Holm–Bonferroni method was used to correct for multiple comparisons. P < 0.05 was considered significant. 
Results
The MG-Specific Rlbp-Cre:Dicer-cKOMG Mouse Is a Reliable Mouse Model Resulting in a Robust Phenotype Resembling Retinal Degenerative Diseases
In order to perform an in vivo in-depth analysis of retinal health and function in the MG-specific Dicer-cKO mouse model (referred to as Rlbp-Cre:Dicer-cKOMG),9 we first validated the establishment of the reported phenotype. Tamoxifen was injected on postnatal days 11 to 14 (P11–P14)9 to activate the Cre recombinase, and the tissue was evaluated histologically 1 and 6 months after Dicer deletion (Fig. 1A). The wildtype showed sufficient reporter induction (∼98%) in glutamine synthetase (GS) and Sox9 expressing MG (Figs. 1B–1G), consistent with previous reports.9,29 The retinas of the 1-month-old Rlbp-Cre:Dicer-cKOMG showed complete reporter induction throughout the retina, as well as the previously reported random areas with somewhat dislocated MG (Figs. 1H–1J). In the retinas of the 6-month-old Dicer-cKOMG mice, severe disruptions and thinning of the central areas were found, predominantly in the ONL, confirming the reported phenotype.9 The MG appeared to form a large glial seal (Figs. 1K, 1L, arrowheads) resembling the events reported in late-stage retinal remodeling30 and had reduced glutamine synthase labeling (Fig. 1M, arrows), a hallmark of specific gliosis.30 Furthermore, the RPE seemed to interact with the ELM/retina (Fig. 1K, arrows), a process that appears to occur in a variety of retinal diseases with photoreceptor loss.31,32 Hence, all previously reported features of this mouse model were present, confirming the robust and reliable phenotype and allowing an in-depth in vivo structural and functional analysis to elaborate the sequence of events leading to this severe degenerative state. 
Figure 1.
 
miRNA loss in MG results in retinal degeneration at late stage. (A) Experimental design showing Cre activation at P11 to P14 and time points of analysis 1 and 6 months after Cre induction (red stars). (BM) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), glutamine synthetase (GS), and SOX9, as well as DAPI nuclear staining of center areas of Rlbp-Cre wildtype (WT) mice (BG) or Rlbp-Cre:Dicer-cKOMG mice (HM), 1 or 6 months after Cre induction. The insets in C, F, I, and L are shown in D, G, J, and M, respectively. Red arrowheads in J indicate displaced MG. Arrows in K show RPE–ELM interactions. Arrowheads in K and L show MG seal formation. Arrows in M show GS-negative cells. Scale bars: 200 µm (B, E, H, K), 50 µm (C, F, I, L), 25 µm (D, G, J, M).
Figure 1.
 
miRNA loss in MG results in retinal degeneration at late stage. (A) Experimental design showing Cre activation at P11 to P14 and time points of analysis 1 and 6 months after Cre induction (red stars). (BM) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), glutamine synthetase (GS), and SOX9, as well as DAPI nuclear staining of center areas of Rlbp-Cre wildtype (WT) mice (BG) or Rlbp-Cre:Dicer-cKOMG mice (HM), 1 or 6 months after Cre induction. The insets in C, F, I, and L are shown in D, G, J, and M, respectively. Red arrowheads in J indicate displaced MG. Arrows in K show RPE–ELM interactions. Arrowheads in K and L show MG seal formation. Arrows in M show GS-negative cells. Scale bars: 200 µm (B, E, H, K), 50 µm (C, F, I, L), 25 µm (D, G, J, M).
miRNA Loss in Müller Glia Does Not Result in Substantial Structural or Functional Retinal Impairment in the Early Phase But Leads to Some Alterations in the ELM–RPE and INL
To investigate structural abnormalities in vivo, we conducted SD-OCT 1 month after Dicer deletion, the time point by which ∼70% to 80% of all miRNAs are reduced9,21 (Fig. 2A). Thickness measurements for whole retinal thickness and the individual layers were conducted in the central areas (∼650-µm radius from the ON, across the nasal–temporal and superior–inferior axes) (Fig. 2D; Supplementary Figs. S1A, S2A). 
Figure 2.
 
Early-stage MG alterations have no impact on retinal structure and function. (A) Experimental design. (B) OCT images of WT and Rlbp-Cre:Dicer-cKOMG (R-cKOMG, cKO) center retinas (1300-µm diameter) at the nasal–temporal axis 1 month after Cre induction. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 6). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 6) 1 month after Cre induction. (F) Estimated saturated amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 2.
 
Early-stage MG alterations have no impact on retinal structure and function. (A) Experimental design. (B) OCT images of WT and Rlbp-Cre:Dicer-cKOMG (R-cKOMG, cKO) center retinas (1300-µm diameter) at the nasal–temporal axis 1 month after Cre induction. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 6). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 6) 1 month after Cre induction. (F) Estimated saturated amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
OCT scans of wildtype and Rlbp-Cre:Dicer-cKOMG 1 month after Cre induction did not show any substantial abnormalities. The overall retinal thickness was about 215 µm for both conditions, which equals the thickness of a normal mouse retina.3335 Some nasal areas in the Rlbp-Cre:Dicer-cKOMG retinas were, however, ∼10% thicker than the wildtype, caused by an enlarged nasal ELM–RPE area (Figs. 2C, 2D; Supplementary Fig. S1A). This suggests a possible dilatation due to a swelling of MG endfeet (ELM enlargement), fluid build-up (or both), or a possible RPE enlargement. Furthermore, the INL was increased (∼10 µm) (Fig. 2D, Supplementary Fig. S1A), an event known to occur in human RP.3638 
Next, we conducted scotopic ERGs but no rod photoreceptor (a-wave amplitudes) or rod bipolar cell (b-wave amplitudes) impairments were present 1 month after Dicer deletion, (Fig. 2E). We then performed an in-depth analysis using the Naka–Rushton equation.39 This equation allows the evaluation of responsiveness of the tissue via the saturated amplitude (Vmax), sensitivity via the semi-saturation constant (K), and heterogeneity via slope (n) (Fig. 2F). Although there was no difference in the responsiveness and sensitivity in the b-wave response of Rlbp-Cre:Dicer-cKOMG mice, the slope was different. This indicates patchy functional impairments, which are known features in early stages of retinal dystrophies with drastic photoreceptor loss such as RP.40,41 
Initial Structural Abnormalities, Indicated by Hyperreflective Foci and Photoreceptor Layer Thinning, Do Not Result in Functional Impairment 3 Months After Dicer Deletion
We next analyzed the retinas of Rlbp-Cre:Dicer-cKOMG mice 3 months after Dicer deletion and compared it to age-matched wildtypes (Fig. 3A). No differences in the overall retinal thickness or the particular layer thicknesses were found (Figs. 3B–3D; Supplementary Figs. S1B, S2B). However, about 40% of all mice analyzed displayed hyperreflective foci in the ELM–RPE or outer plexiform layer (OPL) (Fig. 3B, arrowheads). These foci are often cell accumulations (microglia)19 or small detachments caused by fluid build-up.4245 Because we could not confirm microglia or macrophage accumulation in retinal cross-sections, the foci could be a consequence of the ELM enlargement we found in the 1-month Rlbp-Cre:Dicer-cKOMG mice. Furthermore, despite an overall preserved thickness, there was an initial reduction of the ELM–RPE region in the temporal central cKO retina (Fig. 3D), indicating a possible initial photoceptor impairment. The INL in the 3-month Rlbp-Cre:Dicer-cKOMG mice was back to normal size (Fig. 3D; Supplementary Figs. S1B, S2B). 
Figure 3.
 
Intermediate-stage retinas display structural abnormalities but no functional impairments. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas, 1300-µm diameter) 3 months after Cre induction at the nasal–temporal axis. Arrowheads indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 11). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 10) and Rlbp-Cre:Dicer-cKOMG mice (n = 8). (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 3.
 
Intermediate-stage retinas display structural abnormalities but no functional impairments. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas, 1300-µm diameter) 3 months after Cre induction at the nasal–temporal axis. Arrowheads indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 11). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 10) and Rlbp-Cre:Dicer-cKOMG mice (n = 8). (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Despite these initial structural alterations, scotopic ERGs showed no signs of neuronal impairment (Fig. 3E); however, we found a trend toward a slightly higher amplitude of the scotopic b-wave in the Rlbp-Cre:Dicer-cKOMG retinas. Vmax, semi-saturation K, and slope n did not differ from wildtype retinas. This shows not only no functional impairment at this intermediate stage but also the offset of the differences in heterogeneity found in the early stages, which appear to be transient (Fig. 3F vs. Fig. 2F). 
Dicer Loss in Müller Glia Leads to Significant Neuronal Functional Loss in Outer and Inner Retina at Late Stage
Next, we analyzed the late phase and conducted OCT measurements 6 months after Cre induction (Dicer/miRNA loss). We found a significantly progressed phenotype regarding pathophysiological features and functional impairment (Fig. 4; Supplementary Figs. S1C, S2C). The retinas of the Rlbp-Cre:Dicer-cKOMG mice were thinner and displayed more hyperreflective foci. These foci seemed to be more defined (less cloudy), particularly between the ELM and RPE (Fig. 4B, arrows). Compared to age-matched wildtype mice, retinas of the Rlbp-Cre:Dicer-cKOMG mice displayed an approximately 20% reduction of the overall retinal thickness (Figs. 4C, 4D), independent of orientation. The most prominent reduction was seen in the ELM–RPE and the ONL reflecting photoreceptor loss. Interestingly, the inner layers were unchanged or slightly thicker (Fig. 4D; Supplementary Figs. S1C, S2C). This severe pathological phenotype was accompanied by a significantly reduced scotopic a-wave and b-wave (Fig. 4E). The Vmax (saturated amplitude) was significantly reduced, indicating reduced retinal responsiveness in the 6-month Dicer-cKOMG and potential inner retinal defects (Fig. 4F). 
Figure 4.
 
The miRNA-depleted MG cause photoreceptor loss and vision impairment at the late stage. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas; 1300-µm diameter) 6 months after Cre induction at the nasal–temporal axis. Arrows indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 7). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 5) and Rlbp-Cre:Dicer-cKOMG mice (n = 5) 6 months after Cre induction. (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 4.
 
The miRNA-depleted MG cause photoreceptor loss and vision impairment at the late stage. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas; 1300-µm diameter) 6 months after Cre induction at the nasal–temporal axis. Arrows indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 7). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 5) and Rlbp-Cre:Dicer-cKOMG mice (n = 5) 6 months after Cre induction. (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Immunofluorescent labeling using antibodies against calbindin to label horizontal cells (HCs) and amacrine cells (ACs), as well as calretinin (ACs) and choline acetyltransferase (ChAT, ACs), confirmed inner retinal defects (Fig. 5). These markers also label retinal ganglion cells and displaced ACs in the GCL, but no impairments were seen in this layer (Figs. 5B–5G). We found severe disruptions of the neuronal circuitry in 6-month Rlbp-Cre:Dicer-cKOMG retinas, with some neuronal cell bodies being located beneath the glial seal in the outer retina (Fig. 5E, yellow arrow). Similar events occur during late-stage retinal remodeling in AMD or RP.30,4648 This indicates that the MG-driven retinal degeneration patterns found in the late-stage Rlbp-Cre:Dicer-cKOMG mouse do indeed resemble those of non–glia-based retinal degenerative models. These patterns also include the glial seal formation and the RPE alterations (Figs. 5E–5G, arrowheads and white arrow, respectively). In particular the RPE appeared to grow inside the retina by forming quite noticeable invaginations. These invaginations resembled the shape of the hyperreflective foci seen in the OCT scans (Fig. 5E, inset, red arrow). 
Figure 5.
 
Retinal remodeling in late-stage retinas with miRNA-depleted MG. (A) Experimental design. (BG) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), calretinin (B, E), calbindin (C, F), and ChAT (D, G), as well as DAPI nuclear staining of center areas of WT mice (BD) or Rlbp-Cre:Dicer-cKOMG mice (EG), 6 months after Cre induction. Arrowheads in E, F, and G show a glial seal; the yellow arrow in E indicates a neuronal cell body beneath the glial seal; the red arrow in the high-magnification inset in E shows invagination; the white arrow in F indicates RPE–ELM interaction/fusion. Scale bars: 50 µm. Layer explanation is given in Figure 1.
Figure 5.
 
Retinal remodeling in late-stage retinas with miRNA-depleted MG. (A) Experimental design. (BG) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), calretinin (B, E), calbindin (C, F), and ChAT (D, G), as well as DAPI nuclear staining of center areas of WT mice (BD) or Rlbp-Cre:Dicer-cKOMG mice (EG), 6 months after Cre induction. Arrowheads in E, F, and G show a glial seal; the yellow arrow in E indicates a neuronal cell body beneath the glial seal; the red arrow in the high-magnification inset in E shows invagination; the white arrow in F indicates RPE–ELM interaction/fusion. Scale bars: 50 µm. Layer explanation is given in Figure 1.
Initial Rod Photoreceptor Degeneration Is Observed at 4 Months After miRNA Loss in Müller Glia
To decipher the events happening between the intermediate stage (3 months) and late stage (6 months) and to determine the time point of neuronal cell and neuronal functional loss, we conducted OCT and ERG at 4 and 5 months after Dicer deletion (Fig. 6A). OCT scans of 4-month Rlbp-Cre:Dicer-cKOMG retinas showed many hyperreflective foci (cloudy appearance), predominantly in the space between the ELM and the RPE, reflecting pathogenesis progression (Fig. 6B, arrowheads). Thickness measurements displayed initial retinal thinning in both orientations (Fig. 6C; Supplementary Figs. S1D, S2D, orange graphs). The most affected layer was the ONL (photoreceptor loss) and to a lesser extent in the ELM–RPE area (unchanged compared to the 3-month time point) (Figs. 6D, 6E). 
Figure 6.
 
Rod functional impairments are evident 4 months after MG miRNA depletion. (A) Experimental design. (B, E) OCT images of central and peripheral retinal areas of Rlbp-Cre:Dicer-cKOMG mice 4 months (B) and 5 months (E) after Cre induction. Arrowheads indicate structural impairments, and arrows indicate enlarged INL. (C) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axes of WT mice (n = 11), 4-month mice (n = 5), and 5-month Rlbp-Cre:Dicer-cKOMG mice (n = 5). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (F) Timeline plots showing the averaged thickness of Rlbp-Cre:Dicer-cKOMG mice over time for the total retinal and specific layers. (G) Fullfield scotopic electroretinogram recordings showing a-wave and b-wave amplitudes of 4-month Rlbp-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 15). (H) Estimated maximum amplitudes (Vmax, responsiveness) semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B, E). Layer explanation is given in Figure 1.
Figure 6.
 
Rod functional impairments are evident 4 months after MG miRNA depletion. (A) Experimental design. (B, E) OCT images of central and peripheral retinal areas of Rlbp-Cre:Dicer-cKOMG mice 4 months (B) and 5 months (E) after Cre induction. Arrowheads indicate structural impairments, and arrows indicate enlarged INL. (C) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axes of WT mice (n = 11), 4-month mice (n = 5), and 5-month Rlbp-Cre:Dicer-cKOMG mice (n = 5). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (F) Timeline plots showing the averaged thickness of Rlbp-Cre:Dicer-cKOMG mice over time for the total retinal and specific layers. (G) Fullfield scotopic electroretinogram recordings showing a-wave and b-wave amplitudes of 4-month Rlbp-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 15). (H) Estimated maximum amplitudes (Vmax, responsiveness) semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B, E). Layer explanation is given in Figure 1.
OCT scans from 5-month Rlbp-Cre:Dicer-cKOMG retinas showed less hyperreflective foci in the area between the ELM and RPE (Fig. 6E, arrowheads), suggesting a possible reduction of fluid build-up in this area. However, the retinal lamination appeared to be altered, and in particular the IPL appeared enlarged, at least in some areas (Fig. 6B, arrows). The measurements of the retinal layers showed significant thinning of the total retina, especially of the ONL and the ELM–RPE area, indicating progressed photoreceptor loss (Figs. 6C, 6D, red graphs). A time course of the overall structural alterations in the Dicer-cKO (averaged measurements across the nasal–temporal and superior–inferior axes) is given in Figure 6F. The ELM–RPE area began to decrease 3 months after Dicer deletion (∼35% declined by 6 months compared to the wildtype baseline). This ELM–RPE alteration went hand in hand with the decrease of the ONL thickness, evident 4 months after Dicer deletion (50% reduction by 6 months compared to the wildtype baseline) (Fig. 6F). Conversely, the IPL increased in thickness beginning 4 months after Dicer deletion and maintained this thickness until late stage, despite remodeling or perhaps because of it. The other layers remained unaffected (Supplementary Fig. S3A). 
ERGs conducted 4 months after Dicer deletion showed a significant reduction in the scotopic a-wave amplitude relative to wildtype, confirming a decline in functional integrity of the retina at this stage (Fig. 6G). This loss of rod function correlates with the thinning in ONL and ELM–RPE observed in OCT; however, the scotopic b-wave, an indicator of rod bipolar cell and MG function was not impaired, although a slight trend toward a reduced b-wave response was found (Fig. 6G). The in-depth analysis of the b-wave response using Naka–Rushton equation showed that responsiveness (Vmax), sensitivity (K), and heterogeneity (slope n) were not altered at this stage (Fig. 6H). The time course of the scotopic a-wave and b-wave ranging from 1 to 6 months after Dicer deletion and the time course of the Naka–Rushton parameters are given in Supplementary Figs. S3B and S3C, showing the first functional rod deficit 4 months after Dicer deletion. 
The Loss of Cone Function Precedes the Loss of Rod Function
Because MG play an essential role in cone pigment recycling, we next measured the photopic b-wave response 3, 4, and 6 months after Dicer deletion to analyze cone health in the cKO mouse (Fig. 7A). A photopic a-wave is not detectable in rodents due to a small cone population.49,50 We found that cone loss preceded rod loss; 3 months after Dicer deletion, the photopic b-wave amplitude in the cKO was reduced by ∼20% compared to age-matched wildtypes and further dropped with age (Figs. 7B–7D). Immunofluorescent labeling with antibodies against M opsin showed less dense cone outer segments in cKO retinas that also appeared scattered or fragmented, validating the functional deficit (Figs. 7E–7H’, arrowheads). 
Figure 7.
 
Rlbp-Cre:Dicer-cKO mice display loss of cone function 3 month after Cre induction. (A) Experimental design. (BD) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots for WT and Rlbp-Cre:Dicer-cKOMG mice at 3 months (WT, n = 10; cKO, n = 8) (B), 4 months (WT, n = 15; cKO, n = 5) (C), and 6 months (WT, n = 5; cKO, n = 5) (D). (EH’) Immunofluorescent labeling of cross-sections (E, G) and flatmounts (F/F’, H/H’) with antibodies against RFP (Rlbp1:Tomato) and M opsin (medium-wavelength cone opsin) of center areas of Rlbp-Cre WT mice (E, F) and 3-month Rlbp-Cre:Dicer-cKOMG mice (G, H). Insets in F and H are shown in F’ and H’. Arrowheads indicate areas with reduced and scattered M opsin expression. Values are expressed as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 50 µm (E, G, F’, H’) and 200 µm (F, H). Layer explanation is given in Figure 1.
Figure 7.
 
Rlbp-Cre:Dicer-cKO mice display loss of cone function 3 month after Cre induction. (A) Experimental design. (BD) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots for WT and Rlbp-Cre:Dicer-cKOMG mice at 3 months (WT, n = 10; cKO, n = 8) (B), 4 months (WT, n = 15; cKO, n = 5) (C), and 6 months (WT, n = 5; cKO, n = 5) (D). (EH’) Immunofluorescent labeling of cross-sections (E, G) and flatmounts (F/F’, H/H’) with antibodies against RFP (Rlbp1:Tomato) and M opsin (medium-wavelength cone opsin) of center areas of Rlbp-Cre WT mice (E, F) and 3-month Rlbp-Cre:Dicer-cKOMG mice (G, H). Insets in F and H are shown in F’ and H’. Arrowheads indicate areas with reduced and scattered M opsin expression. Values are expressed as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 50 µm (E, G, F’, H’) and 200 µm (F, H). Layer explanation is given in Figure 1.
The Loss of miRNAs in the RPE Causes Partial RPE Alteration
Because Rlbp1 is expressed not only in MG but also in the RPE, a contribution of a malfunctioning RPE to the observed rod loss in the Dicer-cKOMG mouse cannot be ruled out. We therefore labeled flatmounted RPE with zonula occludens 1 (ZO-1), a marker for tight junctions of the RPE,51 and orthodenticle homeobox 2 (OTX2), a marker known to be expressed in mature RPE.52,53 wildtype and cKO RPE displayed a mosaic expression, in agreement with previous reports.22,54 Some animals had about 70% reporter induction, whereas others had only about 40%. Wildtype RPE cells and reporter negative cells in the cKO were healthy, displaying defined ZO-1–labeled membranes and one or two OTX2+ nuclei in the center of the cell (Figs. 8A, 8A’). 
Figure 8.
 
Rlbp-Cre:Dicer-cKO mice have partially impaired RPE. (AC) Immunofluorescent labeling with antibodies against RFP (Rlbp1-Tomato), ZO-1, OTX2, and DAPI nuclear staining RPE flatmounts of 3-month Rlbp-Cre WT mice (A), 3-month Rlbp-Cre:Dicer-cKO mice (B), and 6-month Rlbp-Cre:Dicer-cKO mice (C). Insets in A to C are shown in A’ to C’. Arrows in B/B’ show altered nuclei locations in the 3-month Rlbp-Cre:Dicer-cKO mice. Arrows in C’ show enlarged RPE cells or RPE cells that lost their ZO-1+ membrane. Arrowheads in C’ show Rlbp-Cre:Dicer-cKO RPE cells with reduced ZO-1 and OTX2 expression. Scale bars: 50 µm (A–C) and 25 µm (A’C’).
Figure 8.
 
Rlbp-Cre:Dicer-cKO mice have partially impaired RPE. (AC) Immunofluorescent labeling with antibodies against RFP (Rlbp1-Tomato), ZO-1, OTX2, and DAPI nuclear staining RPE flatmounts of 3-month Rlbp-Cre WT mice (A), 3-month Rlbp-Cre:Dicer-cKO mice (B), and 6-month Rlbp-Cre:Dicer-cKO mice (C). Insets in A to C are shown in A’ to C’. Arrows in B/B’ show altered nuclei locations in the 3-month Rlbp-Cre:Dicer-cKO mice. Arrows in C’ show enlarged RPE cells or RPE cells that lost their ZO-1+ membrane. Arrowheads in C’ show Rlbp-Cre:Dicer-cKO RPE cells with reduced ZO-1 and OTX2 expression. Scale bars: 50 µm (A–C) and 25 µm (A’C’).
Three-month cKO RPE cells, however, had less intense ZO-1 and OTX2 labeling and dislocated nuclei (toward the cell membrane) (Figs. 8B, 8B’, arrows). These dislocated nuclei appeared to be smaller and were mostly arranged in pairs. Some cells seemed to have several nuclei, suggesting either cell division or a potential nuclear migration. Six-month cKO RPE cells displayed a more progressed phenotype, as many cells displayed very weak ZO-1 expression in the cell membrane and faint OTX2+ or OTX2 nuclei (Figs. 8C, 8C’, arrowheads), indicating impaired RPE. Interestingly, the RPE of cKO mice had also tdTomato (reporter)-negative “normal” RPE cells (mosaic expression). They displayed mostly wildtype-like ZO-1 and OTX2 expression patterns. However, some of these normal (reporter-negative) RPE cells appeared to be enlarged with partly absent ZO-1+ membranes (Fig. 8C, arrows). This may suggest a possible negative influence of adjacent cKO RPE cells. Overall, the partial alteration of the RPE in the Rlbp-Dicer-cKO mouse very likely contributed to observed retinal (rod) degeneration. 
Glast-Cre:Dicer-cKO Mice Display a Similar Disease Phenotype But With More Persevered Retinal Structure
To assess the extent of RPE contribution to the observed phenotype in the Rlbp-Cre:Dicer-cKO mouse, we created the Glast-Cre:Dicer-cKOMG mouse with no reporter expression in the RPE.54 Glast (i.e., glutamate aspartate transporter, encoded by Slc1a3 and also known as EEAT1) is expressed in brain astrocytes55 and MG. We used the same experimental paradigm and validated the reporter induction in 3-month-old mice (Supplementary Fig. S4). RPE and retinal astrocytes were reporter negative in agreement with previous reports.29,54 Retinal cross-sections and flatmounts showed similar induction patterns in MG compared to Rlbp-Cre; however, the efficiency was quite heterogeneous and a little less efficient (Glast-Cre with 70%–90% vs. Rlbp-Cre with ∼98% reporter+ MG of total MG). 
Next, we conducted OCT and ERG analysis of the Glast-CreER wildtype and cKO mice 3 and 6 months after Cre induction (Dicer deletion) to analyze structure and function (Fig. 9A). OCT scans showed that Glast-Cre wildtype mice were not different form Rlbp-Cre or any other wildtype strain. In the 3-month Glast-Cre:Dicer-cKOMG mice, the overall retinal architecture was normal but hyperreflective foci were present in the ELM–RPE region (Fig. 9B, arrowheads; Supplementary Figs. S5A–S5C). This finding suggests that these hyperreflective foci are primarily caused by MG dysfunction and not by RPE impairment. The retinas of 6-month Glast-Cre:Dicer-cKOMG mice were quite heterogeneous (probably due to less efficient induction), had thinner retinas than the wildtype retinas, and displayed more hyperreflective foci between the ELM and RPE compared to the Rlbp-Cre:Dicer-cKOMG retinas at this stage (Figs. 9D, 9E, arrowheads). This thinning was due to a reduction of the ELM–RPE area and the ONL, hence affecting photoreceptors. Overall, although the Rlbp-Dicer-cKO strain appeared more affected (ONL), both cKO strains resulted in similar thinning (Figs. 9E, 9F; Supplementary Figs. S5D, S5E). 
Figure 9.
 
Glast-Cre:Dicer-cKO mice display hyperreflective foci and retinal thinning. (A) Experimental design. (B) OCT images of WT and or Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 3 months after Cre induction. (C) Immunofluorescent labeling of Glast-Cre:Dicer-cKO RPE flatmounts 3 months after Cre induction with antibodies against RFP (Glast:Tomato), ZO-1, OTX2, and DAPI. (D) OCT images of Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 6 months after Cre induction. Arrowheads in B and D indicate structural impairments. (E) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axis of WT mice (n = 5) and Glast-Cre:Dicer-cKOMG mice (n = 6) 6 months after Cre induction, in comparison to age-matched Rlbp-Cre:Dicer-cKOMG mice (n = 7). (F) Spider plots of the thickness (µm) of the retinal layers for WT and Glast-Cre:Dicer-cKOMG mice, in comparison to Rlbp-Cre:Dicer-cKOMG mice. Values are expressed as mean ± SD. Significant differences are indicated. *Wildtype-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (BD) and 25 µm (inset). Layer explanation is given in Figure 1.
Figure 9.
 
Glast-Cre:Dicer-cKO mice display hyperreflective foci and retinal thinning. (A) Experimental design. (B) OCT images of WT and or Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 3 months after Cre induction. (C) Immunofluorescent labeling of Glast-Cre:Dicer-cKO RPE flatmounts 3 months after Cre induction with antibodies against RFP (Glast:Tomato), ZO-1, OTX2, and DAPI. (D) OCT images of Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 6 months after Cre induction. Arrowheads in B and D indicate structural impairments. (E) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axis of WT mice (n = 5) and Glast-Cre:Dicer-cKOMG mice (n = 6) 6 months after Cre induction, in comparison to age-matched Rlbp-Cre:Dicer-cKOMG mice (n = 7). (F) Spider plots of the thickness (µm) of the retinal layers for WT and Glast-Cre:Dicer-cKOMG mice, in comparison to Rlbp-Cre:Dicer-cKOMG mice. Values are expressed as mean ± SD. Significant differences are indicated. *Wildtype-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (BD) and 25 µm (inset). Layer explanation is given in Figure 1.
Glast-Cre:Dicer-cKO Display Impaired Cone Function But More Preserved Rod Function
Because the first neuronal dysfunctions in the Rlbp-Cre:Dicer-cKOMG mouse concerned cones, we next conducted photopic ERG recordings in the Glast-Cre:Dicer-cKOMG mouse 3 and 6 months after Dicer deletion. Despite unaltered retinal structure, we found cone impairment 3 months after Dicer deletion, which further progressed over time (Figs. 10A, 10B). M opsin expression appeared scattered and fragmented, displaying the same phenotype as the Rlbp-Cre:Dicer-cKOMG mouse (Figs. 10C, 10D vs. Fig. 7). Because both MG Dicer-cKOMG strains resulted in the same outcome, the cone functional loss appears to be due to molecular (miRNA) alterations in MG. 
Figure 10.
 
Glast-Cre:Dicer-cKO mice show loss of cone function 3 months after Dicer deletion. (A, B) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots of 3-month Glast-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 5) (A) and 6-month Glast-Cre:Dicer-cKOMG mice (n = 6) in comparison to WT mice (n = 5) (B). (C, D) Immunofluorescent labeling of retinal cross-sections with antibodies against RFP (Glast:tdTomato) and M opsin (medium-wavelength cone opsin) of center areas of WT mice (C) and 3-month Glast-Cre:Dicer-cKOMG mice (D). (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of Glast-Cre:Dicer-cKOMG (n = 6) in comparison to WT (n = 5) and Rlbp-Cre:Dicer-cKOMG (n = 5). (F, F’) Immunofluorescent labeling of central retinal areas of the Glast-Cre:Dicer-cKOMG mouse 6 months after Dicer deletion, with antibodies against RFP (Glast:Tomato), GS, and SOX9, as well as DAPI nuclear staining. Arrows in F show a disturbed ELM, arrowheads in F’ display GS-reduced MG. Scale bars: 50 µm (C, D, F) and 25 µm (F’). Values are expressed as mean ± SEM. Significant differences are indicated. *WT-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Layer explanation is given in Figure 1.
Figure 10.
 
Glast-Cre:Dicer-cKO mice show loss of cone function 3 months after Dicer deletion. (A, B) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots of 3-month Glast-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 5) (A) and 6-month Glast-Cre:Dicer-cKOMG mice (n = 6) in comparison to WT mice (n = 5) (B). (C, D) Immunofluorescent labeling of retinal cross-sections with antibodies against RFP (Glast:tdTomato) and M opsin (medium-wavelength cone opsin) of center areas of WT mice (C) and 3-month Glast-Cre:Dicer-cKOMG mice (D). (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of Glast-Cre:Dicer-cKOMG (n = 6) in comparison to WT (n = 5) and Rlbp-Cre:Dicer-cKOMG (n = 5). (F, F’) Immunofluorescent labeling of central retinal areas of the Glast-Cre:Dicer-cKOMG mouse 6 months after Dicer deletion, with antibodies against RFP (Glast:Tomato), GS, and SOX9, as well as DAPI nuclear staining. Arrows in F show a disturbed ELM, arrowheads in F’ display GS-reduced MG. Scale bars: 50 µm (C, D, F) and 25 µm (F’). Values are expressed as mean ± SEM. Significant differences are indicated. *WT-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Layer explanation is given in Figure 1.
Scotopic ERG recordings 3 and 4 months after Dicer deletion showed no rod photoreceptor impairment, in contrast to the observations in the Rlbp-Cre:Dicer-cKOMG mouse (Supplementary Figs. S6A, S6B). Naka–Rushton analysis also showed no alterations except an increased Vmax (responsiveness) in the 4-month Glast-Cre:Dicer-cKOMG mouse compared to the wildtype. As mentioned before, a similar trend was seen in the Rlbp-Cre:Dicer-cKOMG mouse (Supplementary Fig. S6B vs. Fig. S63E). 
Six-month Glast-Cre:Dicer-cKOMG retinas displayed rod dysfunction (scotopic a-wave) leading also to initial functional loss in the inner retina (scotopic b-wave) (Fig. 10E, Supplementary Figs. S6C). Overall, the scotopic functional loss in the 6-month Glast-Cre:Dicer-cKOMG mouse resembled the functional loss found in the 6-month Rlbp-Cre:Dicer-cKOMG mouse. However, the inner retina (rod bipolar cells) in the 6-month Glast-Cre:Dicer-cKOMG were less affected (unchanged Vmax) (Supplementary Fig. S6C). Histological analysis showed that Glast-Cre:Dicer-cKOMG retinas also had areas with massive photoreceptor loss and ELM impairments (Figs. 10F–10H, arrows). In addition, MG in the 6-month Glast-Dicer-cKOMG had reduced or absent glutamine synthetase labeling (Figs. 10F’, arrowheads). 
Discussion
Many retinal dystrophies are not yet fully understood. Although retinitis pigmentosa or macular degenerations have predominantly genetic causes primarily affecting the RPE and photoreceptors, there is growing evidence that MG not only are responders to tissue changes but also contribute to disease progression5661 or might even participate in the initiation of degenerative events.19,20,6264 
MG have a specific set of miRNAs different from those of neurons.29 The loss of these MG-specific miRNAs in young glia results in abnormal glial behavior and a slow but severe photoceptor loss with subsequent retinal remodeling,9 as it occurs in RP or AMD.46,6568 Therefore, any pathologies seen after manipulation are caused by malfunctioning MG, making this mouse a very interesting model for studying MG-induced retinal disease phenotypes. The onset and the order of events of the observed neurodegeneration, as well as whether the RPE contributes to the phenotype, were not known and were investigated in this study. 
Here, we have shown that MG dysfunction, caused by the deletion of Dicer and mature miRNAs in the glia, leads first to impairments of cone health (summarized in Fig. 11). The first pathological indications are found in the ELM–RPE area. Structural and functional cone impairment was evident in two different MG reporter lines (i.e. Rlbp-Cre or Glast-Cre), 3 months after manipulation, validating this robust phenotype. Retinas of both strains displayed hyperreflective foci, which are considered indicators for disease,69 in particular in AMD attributed to ectopic RPE.7072 Our Dicer-cKO model now suggests that hyperreflective foci can also be attributed to MG malfunction. Furthermore, cone impairment was followed by rod impairment, which was more accelerated in the Rlbp-Cre–driven strain, due to partial RPE malfunction. Therefore, the Glast-Cre strain allows the study of MG-driven events, and the Rlbp-Cre strain additionally allows the study of RPE-related events and RPE–MG interactions. 
Figure 11.
 
Dicer-depleted MG lead to cone functional loss with subsequent rod loss. Schematic overview of the major events happening in the MG/RPE-driven retinal degeneration model (Rlbp-CreER:Dicer-cKO) and the MG-driven retinal degeneration model (Glast-CreER:Dicer-cKO) emphasizing the shared pathological features: cone outer segment impairment and cone functional loss and hyperreflective foci in the ELM–RPE at the intermediate state and rod degeneration/rod functional loss and interneuron degeneration leading to retinal remodeling at the late stage. Additional features evident in both models include ELM breakage and glial seal formation. The Rlbp-CreER:Dicer-cKO model displayed furthermore impaired RPE contributing to rod loss and accelerating the overall pathological phenotype.
Figure 11.
 
Dicer-depleted MG lead to cone functional loss with subsequent rod loss. Schematic overview of the major events happening in the MG/RPE-driven retinal degeneration model (Rlbp-CreER:Dicer-cKO) and the MG-driven retinal degeneration model (Glast-CreER:Dicer-cKO) emphasizing the shared pathological features: cone outer segment impairment and cone functional loss and hyperreflective foci in the ELM–RPE at the intermediate state and rod degeneration/rod functional loss and interneuron degeneration leading to retinal remodeling at the late stage. Additional features evident in both models include ELM breakage and glial seal formation. The Rlbp-CreER:Dicer-cKO model displayed furthermore impaired RPE contributing to rod loss and accelerating the overall pathological phenotype.
First Pathological Indications After Conditional Loss of Dicer and Mature miRNAs in MG Are Found in the ELM–RPE
As early as 1 month after Dicer deletion, we found a thicker ELM–RPE. The ELM, formed by tight and adherens junctions of MG and photoreceptors, plays a substantial role in disease manifestation and progression. An intact ELM is a requirement for an intact ellipsoid zone, and the integrity of both structures is vital for the preservation of normal vision. The ELM represents a barrier for macromolecules and is a structure formed by junctional complexes between inner photoreceptor segments and the outer processes of MG.7376 Alterations in the ELM with subsequent changes in the ellipsoid zone have been reported in diabetic retinopathy7781 and Stargardt disease.61,82,83 With regard to diabetic retinopathy characterized by diabetic macular edema, it was reported that MG undergo hypertrophy. Their swollen endfeet lose their connection to the photoreceptors inner segments due to a decline in occludin,76 a transmembrane protein that contributes to tight junction stabilization and optimal barrier function.84 This disruption then leads to the accumulation of intraretinal fluid, primarily in the inner and outer plexiform layers, and manifests as retinal thickening and hyperreflective loci. 
Furthermore, it is suggested that vascular endothelial growth factor (VEGF) alters tight junctions and promotes vascular permeability in the central nervous system, including the neural retina. Although the mechanisms of this barrier regulation are not yet fully understood, it was reported that VEGF induces phosphorylation-dependent occludin ubiquitination, which is necessary for increased permeability to macromolecules and ions.85 MG are known to express VEGF86,87 and therefore have been suspected of contributing to manifestation of the events seen in diabetic retinopathy.8890 The suspicion that MG are one of or even the driving components in these diseases was confirmed by MG ablation studies. The partial elimination (absence) of MG resulted in the same pathophysiological events.19,20 In our study, which is solely based on Dicer deletion with subsequent mature miRNAs depletion in the glia, we found these hallmarks suggesting that MG miRNAs might regulate these events. It was shown that miRNAs that directly target VEGF (3′UTR) include miR-16, miR-20a, miR-29b, miR-125, miR15c, and miR-200c,9196 and some of them are expressed in MG.29 
Moreover, MG have been shown to actively contribute to absorbing excess fluid from the subretinal space via the water pore aquaporin-4 (AQP-4).97,98 Two miRNAs, miR-29b and miR-320a, were identified as AQP-4 suppressors. miR-29 was found downregulated after stroke/cerebral ischemia, and its overexpression reduced the disruption of the blood–brain barrier after ischemic stroke by downregulating AQP-4.99 Similarly, overexpression of miR-320a in the retina resulted in AQP-4 downregulation and attenuated hypoxia-induced injury including retinal edema.100 A compromised MG function due to miRNA depletion might also cause an alteration in aquaporin expression causing liquid accumulation. 
As mentioned before, ELM disruption can lead to fluid accumulation resulting in retinal thickening and hyperreflective loci. In our two independent MG Dicer cKO models (Rlbp-Cre and Glast-Cre), we found hyperreflective foci in the ELM–RPE area 3 months after Dicer/miRNA depletion. Hyperreflective foci belong to pathological features found in diabetic retinopathy/diabetic macular edema,42 RP,4345 Stargardt disease,61,82,83 and macular telangiectasia101 and are a consequence of ELM impairment. Hyperreflective foci can also be detected in AMD, where they represent deposits rather than edema.70,102,103 Because these foci were found in both of our cKO models (Rlbp-Cre and Glast-Cre), it stands to reason that they are predominantly a consequence of MG malfunction and less due to RPE malfunction. This conclusion is in agreement with two previous independent MG ablation studies in which the RPE was not affected.19,20 This finding suggests that some retinal dystrophies and degenerative diseases, in particular the ones with unknown (non-genetic) origin, might be caused by imbalanced MG miRNAs. Furthermore, the fact that the same pathophysiological ELM–ellipsoid zone alterations were seen in animal models of retinal dystrophies45,104 and ischemia,105 which are models in in which solely neurons are targeted, further supports the hypothesis that the driving forces for these events are the glia. 
A Decline in Cone Photoreceptor Function Precedes Rod Dysfunction
Müller glia have myriad functions in the healthy retina, one of which is cone pigment recycling.18,106109 We found impaired cone photoreceptor structure and function in both MG-specific Dicer-cKO strains, suggesting a central role for MG miRNAs in maintaining cone health in the retina. Cones are the photoreceptors involved in daylight and color vision, and maintaining cone health is crucial to maintaining proper visual function. Loss of cones and cone function is a feature of some retinal diseases such as AMD,110,111 Leber's congenital amaurosis,112,116 and Stargardt disease.61,113,114 The cell type predominantly driving the retinal visual cycle is the MG.107,115 MG generate 11-cis-retinol from the all-trans form via the enzyme cellular retinaldehyde binding protein (CRALBP), encoded by Rlbp1.116119 Hence, MG play a substantial role in visual pigment regeneration. Although the direct link between MG miRNAs and cone function in this model remains unknown and requires a subsequent investigation, it has been shown that loss or reduction of CRALBP expression in MG results in dislocated M opsin and reduced cone function.109,120 Furthermore, it has been shown that peripheral regions in the RD1 retinitis pigmentosa mouse model harbor functional cones and that their prolonged survival is ensured by retinoic acid signaling of peripheral MG.121 This suggests that MG miRNAs might play a role in regulating the MG-driven visual cycle. 
Dicer/miRNA Loss in MG Leads to Rod Loss, With Partial Dicer Deletion in the RPE Accelerating the Process
Both MG-specific Dicer cKO strains resulted in rod loss accompanied by functional impairments that later on affected the inner retina. The overall progression of rod loss was very slow, but the pattern resembled the events found in rodent models of RP122124 and in the early stages of human forms of RP125 caused by genetic defects. This suggests not only that MG contribute to the retinitis pigmentosa phenotype but also that MG miRNAs seem to regulate their behavior. Furthermore, we here utilized the Naka–Rushton equation, an analysis predominantly performed on patient ERGs, that allows an in-depth analysis of the b-wave with regard to responsiveness (Vmax), sensitivity (K), and heterogeneity (slope n).3941 Overall, our Dicer-cKOs displayed similarities comparable to observations made in patient ERGs.125 These similarities include a change in slope at early stages indicative for a patchy onset of functional loss.40,41 This is followed by a reduction in responsiveness of rod bipolar cells due to a reduced rod input126 but the sensitivity (basically the function of the cells) is stable during the intermediate phase (4 months). Finally, at the late stage (6 months), rod bipolar cells display a reduced responsiveness and a decline in sensitivity associated with retinal remodeling, affecting all inner neurons as it occurs at late stages of disease.30,4648 The similarities between these disease studies and our model suggest that, in addition to direct damage to photoreceptors or RPE, as occurs in many inherited RP forms, impaired or malfunctioning MG are very likely contributing substantially to the overall disease progression. More importantly, we showed that these pathological events were not caused by MG absence19,20 but rather miRNA imbalances in the glia. 
However, although cone impairments occurred at the same stage after Dicer loss, the rod loss was more accelerated in the Rlbp-Cre–driven strain that had a partially impaired RPE. The RPE has a variety of pivotal functions required for photoreceptor health and overall retinal health, including continuous renewal of the photoreceptor outer segments, isomerization of all-trans-retinal to 11-cis-retinal, and formation of the blood–retinal barrier.127129 Because of all these vital functions, impairment of the RPE not only accompanies but can also lead to photoreceptor degeneration.3,128,130,131 
Because miRNAs play a significant role in regulating essential biological processes by targeting networks of functionally correlated genes, they are vital components of the molecular networks in the RPE.132,133 In fact, RPE Dicer-cKO studies, as well as studies on selected miRNAs, have shown that RPE miRNAs are required for proper RPE maturation and function.6,7,10,130,134139 
In the Rlbp-Dicer-cKO, we found obvious changes in Dicer miRNA-deprived RPE, including disrupted expression of ZO-1, dislocation of RPE nuclei, and enlarged cell size. Altered ZO-1 expression and enlarged cell size are a known pathophysiological feature indicating RPE malfunction.140143 More importantly, drastically altered ZO-1 expression has been reported after Dicer deletion,7,10 as well as after DiGeorge syndrome chromosomal region 8 (DGCR8) deletion, confirming that not just Dicer loss per se but also miRNA can cause RPE alteration.7 DGCR8 is a part of the Drosha complex that generates precursor miRNAs from primary miRNA transcripts. This study also showed that the observed alterations were due to miRNA loss and not due to Alu element accumulations, which are known to contribute to photoreceptor loss in AMD.7,144 Because Alu elements were also absent in the datasets obtained from FACS-purified MG from the Rlbp-CreER:Dicer-cKOMG mice, it is rather unlikely that they play a substantial role in the observed phenotypes.9,21 
Together, our data show that dysregulated but physically still present MG lead to cone impairment in an otherwise healthy retina. This suggests an additional or even new prospect for the development of retinal diseases, particularly cone dystrophies of unknown origin. Furthermore, our findings indicate that drastic molecular changes long precede histological and functional changes. Histological manifestation (clinical observation) occurs relatively late, which might be the reason for difficulties in treating such complex diseases. 
Acknowledgments
The authors thank Monica Andrade for her assistance with this study. 
Supported by a grant from the New York Empire Innovation Program (SGW); grants from the National Eye Institute, National Institutes of Health (R01 EY032532 to SGW; R01 EY024373 and R21 EY032724 to SF); SUNY Startup Funds (SGW); SUNY Graduate Assistantship (DL, SK, SC); National Eye Institute T35 award (AMR); Catalyst Award from Research to Prevent Blindness Inc./American Macular Degeneration Foundation, an unrestricted award to the Department of Ophthalmology and Visual Sciences at Vanderbilt University Medical Center from Research to Prevent Blindness, Inc. (SF). 
Disclosure: D. Larbi, None; A.M. Rief, None; S. Kang, None; S. Chen, None; K. Batsuuri, None; S. Fuhrmann, None; S. Viswanathan, None; S.G. Wohl, None 
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Figure 1.
 
miRNA loss in MG results in retinal degeneration at late stage. (A) Experimental design showing Cre activation at P11 to P14 and time points of analysis 1 and 6 months after Cre induction (red stars). (BM) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), glutamine synthetase (GS), and SOX9, as well as DAPI nuclear staining of center areas of Rlbp-Cre wildtype (WT) mice (BG) or Rlbp-Cre:Dicer-cKOMG mice (HM), 1 or 6 months after Cre induction. The insets in C, F, I, and L are shown in D, G, J, and M, respectively. Red arrowheads in J indicate displaced MG. Arrows in K show RPE–ELM interactions. Arrowheads in K and L show MG seal formation. Arrows in M show GS-negative cells. Scale bars: 200 µm (B, E, H, K), 50 µm (C, F, I, L), 25 µm (D, G, J, M).
Figure 1.
 
miRNA loss in MG results in retinal degeneration at late stage. (A) Experimental design showing Cre activation at P11 to P14 and time points of analysis 1 and 6 months after Cre induction (red stars). (BM) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), glutamine synthetase (GS), and SOX9, as well as DAPI nuclear staining of center areas of Rlbp-Cre wildtype (WT) mice (BG) or Rlbp-Cre:Dicer-cKOMG mice (HM), 1 or 6 months after Cre induction. The insets in C, F, I, and L are shown in D, G, J, and M, respectively. Red arrowheads in J indicate displaced MG. Arrows in K show RPE–ELM interactions. Arrowheads in K and L show MG seal formation. Arrows in M show GS-negative cells. Scale bars: 200 µm (B, E, H, K), 50 µm (C, F, I, L), 25 µm (D, G, J, M).
Figure 2.
 
Early-stage MG alterations have no impact on retinal structure and function. (A) Experimental design. (B) OCT images of WT and Rlbp-Cre:Dicer-cKOMG (R-cKOMG, cKO) center retinas (1300-µm diameter) at the nasal–temporal axis 1 month after Cre induction. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 6). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 6) 1 month after Cre induction. (F) Estimated saturated amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 2.
 
Early-stage MG alterations have no impact on retinal structure and function. (A) Experimental design. (B) OCT images of WT and Rlbp-Cre:Dicer-cKOMG (R-cKOMG, cKO) center retinas (1300-µm diameter) at the nasal–temporal axis 1 month after Cre induction. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 6). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 6) 1 month after Cre induction. (F) Estimated saturated amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 3.
 
Intermediate-stage retinas display structural abnormalities but no functional impairments. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas, 1300-µm diameter) 3 months after Cre induction at the nasal–temporal axis. Arrowheads indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 11). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 10) and Rlbp-Cre:Dicer-cKOMG mice (n = 8). (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 3.
 
Intermediate-stage retinas display structural abnormalities but no functional impairments. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas, 1300-µm diameter) 3 months after Cre induction at the nasal–temporal axis. Arrowheads indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 7) and Rlbp-Cre:Dicer-cKOMG mice (n = 11). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 10) and Rlbp-Cre:Dicer-cKOMG mice (n = 8). (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 4.
 
The miRNA-depleted MG cause photoreceptor loss and vision impairment at the late stage. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas; 1300-µm diameter) 6 months after Cre induction at the nasal–temporal axis. Arrows indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 7). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 5) and Rlbp-Cre:Dicer-cKOMG mice (n = 5) 6 months after Cre induction. (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 4.
 
The miRNA-depleted MG cause photoreceptor loss and vision impairment at the late stage. (A) Experimental design. (B) OCT images of WT or Rlbp-Cre:Dicer-cKOMG mice (R-cKOMG, cKO, center retinas; 1300-µm diameter) 6 months after Cre induction at the nasal–temporal axis. Arrows indicate hyperreflective foci. (C) Spider plots of the overall retinal thickness (diameter, µm) measured at the nasal–temporal and superior–inferior axes of WT mice (n = 4) and Rlbp-Cre:Dicer-cKOMG mice (n = 7). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of WT mice (n = 5) and Rlbp-Cre:Dicer-cKOMG mice (n = 5) 6 months after Cre induction. (F) Estimated maximum amplitudes (Vmax, responsiveness), semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B). Layer explanation is given in Figure 1.
Figure 5.
 
Retinal remodeling in late-stage retinas with miRNA-depleted MG. (A) Experimental design. (BG) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), calretinin (B, E), calbindin (C, F), and ChAT (D, G), as well as DAPI nuclear staining of center areas of WT mice (BD) or Rlbp-Cre:Dicer-cKOMG mice (EG), 6 months after Cre induction. Arrowheads in E, F, and G show a glial seal; the yellow arrow in E indicates a neuronal cell body beneath the glial seal; the red arrow in the high-magnification inset in E shows invagination; the white arrow in F indicates RPE–ELM interaction/fusion. Scale bars: 50 µm. Layer explanation is given in Figure 1.
Figure 5.
 
Retinal remodeling in late-stage retinas with miRNA-depleted MG. (A) Experimental design. (BG) Immunofluorescent labeling with antibodies against RFP (Rlbp1:Tomato), calretinin (B, E), calbindin (C, F), and ChAT (D, G), as well as DAPI nuclear staining of center areas of WT mice (BD) or Rlbp-Cre:Dicer-cKOMG mice (EG), 6 months after Cre induction. Arrowheads in E, F, and G show a glial seal; the yellow arrow in E indicates a neuronal cell body beneath the glial seal; the red arrow in the high-magnification inset in E shows invagination; the white arrow in F indicates RPE–ELM interaction/fusion. Scale bars: 50 µm. Layer explanation is given in Figure 1.
Figure 6.
 
Rod functional impairments are evident 4 months after MG miRNA depletion. (A) Experimental design. (B, E) OCT images of central and peripheral retinal areas of Rlbp-Cre:Dicer-cKOMG mice 4 months (B) and 5 months (E) after Cre induction. Arrowheads indicate structural impairments, and arrows indicate enlarged INL. (C) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axes of WT mice (n = 11), 4-month mice (n = 5), and 5-month Rlbp-Cre:Dicer-cKOMG mice (n = 5). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (F) Timeline plots showing the averaged thickness of Rlbp-Cre:Dicer-cKOMG mice over time for the total retinal and specific layers. (G) Fullfield scotopic electroretinogram recordings showing a-wave and b-wave amplitudes of 4-month Rlbp-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 15). (H) Estimated maximum amplitudes (Vmax, responsiveness) semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B, E). Layer explanation is given in Figure 1.
Figure 6.
 
Rod functional impairments are evident 4 months after MG miRNA depletion. (A) Experimental design. (B, E) OCT images of central and peripheral retinal areas of Rlbp-Cre:Dicer-cKOMG mice 4 months (B) and 5 months (E) after Cre induction. Arrowheads indicate structural impairments, and arrows indicate enlarged INL. (C) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axes of WT mice (n = 11), 4-month mice (n = 5), and 5-month Rlbp-Cre:Dicer-cKOMG mice (n = 5). (D) Spider plots of the thickness (µm) of the ELM–RPE, ONL, INL, and IPL. (F) Timeline plots showing the averaged thickness of Rlbp-Cre:Dicer-cKOMG mice over time for the total retinal and specific layers. (G) Fullfield scotopic electroretinogram recordings showing a-wave and b-wave amplitudes of 4-month Rlbp-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 15). (H) Estimated maximum amplitudes (Vmax, responsiveness) semi-saturation (K, sensitivity), and slope (n, heterogeneity) using the Naka–Rushton equation. OCT shown as mean ± SD; ERG shown as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (B, E). Layer explanation is given in Figure 1.
Figure 7.
 
Rlbp-Cre:Dicer-cKO mice display loss of cone function 3 month after Cre induction. (A) Experimental design. (BD) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots for WT and Rlbp-Cre:Dicer-cKOMG mice at 3 months (WT, n = 10; cKO, n = 8) (B), 4 months (WT, n = 15; cKO, n = 5) (C), and 6 months (WT, n = 5; cKO, n = 5) (D). (EH’) Immunofluorescent labeling of cross-sections (E, G) and flatmounts (F/F’, H/H’) with antibodies against RFP (Rlbp1:Tomato) and M opsin (medium-wavelength cone opsin) of center areas of Rlbp-Cre WT mice (E, F) and 3-month Rlbp-Cre:Dicer-cKOMG mice (G, H). Insets in F and H are shown in F’ and H’. Arrowheads indicate areas with reduced and scattered M opsin expression. Values are expressed as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 50 µm (E, G, F’, H’) and 200 µm (F, H). Layer explanation is given in Figure 1.
Figure 7.
 
Rlbp-Cre:Dicer-cKO mice display loss of cone function 3 month after Cre induction. (A) Experimental design. (BD) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots for WT and Rlbp-Cre:Dicer-cKOMG mice at 3 months (WT, n = 10; cKO, n = 8) (B), 4 months (WT, n = 15; cKO, n = 5) (C), and 6 months (WT, n = 5; cKO, n = 5) (D). (EH’) Immunofluorescent labeling of cross-sections (E, G) and flatmounts (F/F’, H/H’) with antibodies against RFP (Rlbp1:Tomato) and M opsin (medium-wavelength cone opsin) of center areas of Rlbp-Cre WT mice (E, F) and 3-month Rlbp-Cre:Dicer-cKOMG mice (G, H). Insets in F and H are shown in F’ and H’. Arrowheads indicate areas with reduced and scattered M opsin expression. Values are expressed as mean ± SEM. Significant differences are indicated. *P ≤ 0.05, Mann–Whitney U test. Scale bars: 50 µm (E, G, F’, H’) and 200 µm (F, H). Layer explanation is given in Figure 1.
Figure 8.
 
Rlbp-Cre:Dicer-cKO mice have partially impaired RPE. (AC) Immunofluorescent labeling with antibodies against RFP (Rlbp1-Tomato), ZO-1, OTX2, and DAPI nuclear staining RPE flatmounts of 3-month Rlbp-Cre WT mice (A), 3-month Rlbp-Cre:Dicer-cKO mice (B), and 6-month Rlbp-Cre:Dicer-cKO mice (C). Insets in A to C are shown in A’ to C’. Arrows in B/B’ show altered nuclei locations in the 3-month Rlbp-Cre:Dicer-cKO mice. Arrows in C’ show enlarged RPE cells or RPE cells that lost their ZO-1+ membrane. Arrowheads in C’ show Rlbp-Cre:Dicer-cKO RPE cells with reduced ZO-1 and OTX2 expression. Scale bars: 50 µm (A–C) and 25 µm (A’C’).
Figure 8.
 
Rlbp-Cre:Dicer-cKO mice have partially impaired RPE. (AC) Immunofluorescent labeling with antibodies against RFP (Rlbp1-Tomato), ZO-1, OTX2, and DAPI nuclear staining RPE flatmounts of 3-month Rlbp-Cre WT mice (A), 3-month Rlbp-Cre:Dicer-cKO mice (B), and 6-month Rlbp-Cre:Dicer-cKO mice (C). Insets in A to C are shown in A’ to C’. Arrows in B/B’ show altered nuclei locations in the 3-month Rlbp-Cre:Dicer-cKO mice. Arrows in C’ show enlarged RPE cells or RPE cells that lost their ZO-1+ membrane. Arrowheads in C’ show Rlbp-Cre:Dicer-cKO RPE cells with reduced ZO-1 and OTX2 expression. Scale bars: 50 µm (A–C) and 25 µm (A’C’).
Figure 9.
 
Glast-Cre:Dicer-cKO mice display hyperreflective foci and retinal thinning. (A) Experimental design. (B) OCT images of WT and or Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 3 months after Cre induction. (C) Immunofluorescent labeling of Glast-Cre:Dicer-cKO RPE flatmounts 3 months after Cre induction with antibodies against RFP (Glast:Tomato), ZO-1, OTX2, and DAPI. (D) OCT images of Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 6 months after Cre induction. Arrowheads in B and D indicate structural impairments. (E) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axis of WT mice (n = 5) and Glast-Cre:Dicer-cKOMG mice (n = 6) 6 months after Cre induction, in comparison to age-matched Rlbp-Cre:Dicer-cKOMG mice (n = 7). (F) Spider plots of the thickness (µm) of the retinal layers for WT and Glast-Cre:Dicer-cKOMG mice, in comparison to Rlbp-Cre:Dicer-cKOMG mice. Values are expressed as mean ± SD. Significant differences are indicated. *Wildtype-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (BD) and 25 µm (inset). Layer explanation is given in Figure 1.
Figure 9.
 
Glast-Cre:Dicer-cKO mice display hyperreflective foci and retinal thinning. (A) Experimental design. (B) OCT images of WT and or Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 3 months after Cre induction. (C) Immunofluorescent labeling of Glast-Cre:Dicer-cKO RPE flatmounts 3 months after Cre induction with antibodies against RFP (Glast:Tomato), ZO-1, OTX2, and DAPI. (D) OCT images of Glast-Cre:Dicer-cKOMG center at the nasal–temporal axis 6 months after Cre induction. Arrowheads in B and D indicate structural impairments. (E) Spider plots of the overall retinal thickness at the nasal–temporal and superior–inferior axis of WT mice (n = 5) and Glast-Cre:Dicer-cKOMG mice (n = 6) 6 months after Cre induction, in comparison to age-matched Rlbp-Cre:Dicer-cKOMG mice (n = 7). (F) Spider plots of the thickness (µm) of the retinal layers for WT and Glast-Cre:Dicer-cKOMG mice, in comparison to Rlbp-Cre:Dicer-cKOMG mice. Values are expressed as mean ± SD. Significant differences are indicated. *Wildtype-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Scale bars: 200 µm (BD) and 25 µm (inset). Layer explanation is given in Figure 1.
Figure 10.
 
Glast-Cre:Dicer-cKO mice show loss of cone function 3 months after Dicer deletion. (A, B) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots of 3-month Glast-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 5) (A) and 6-month Glast-Cre:Dicer-cKOMG mice (n = 6) in comparison to WT mice (n = 5) (B). (C, D) Immunofluorescent labeling of retinal cross-sections with antibodies against RFP (Glast:tdTomato) and M opsin (medium-wavelength cone opsin) of center areas of WT mice (C) and 3-month Glast-Cre:Dicer-cKOMG mice (D). (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of Glast-Cre:Dicer-cKOMG (n = 6) in comparison to WT (n = 5) and Rlbp-Cre:Dicer-cKOMG (n = 5). (F, F’) Immunofluorescent labeling of central retinal areas of the Glast-Cre:Dicer-cKOMG mouse 6 months after Dicer deletion, with antibodies against RFP (Glast:Tomato), GS, and SOX9, as well as DAPI nuclear staining. Arrows in F show a disturbed ELM, arrowheads in F’ display GS-reduced MG. Scale bars: 50 µm (C, D, F) and 25 µm (F’). Values are expressed as mean ± SEM. Significant differences are indicated. *WT-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Layer explanation is given in Figure 1.
Figure 10.
 
Glast-Cre:Dicer-cKO mice show loss of cone function 3 months after Dicer deletion. (A, B) Fullfield photopic ERG recordings showing representative ERG waveforms and b-wave intensity–amplitude plots of 3-month Glast-Cre:Dicer-cKOMG mice (n = 5) in comparison to WT mice (n = 5) (A) and 6-month Glast-Cre:Dicer-cKOMG mice (n = 6) in comparison to WT mice (n = 5) (B). (C, D) Immunofluorescent labeling of retinal cross-sections with antibodies against RFP (Glast:tdTomato) and M opsin (medium-wavelength cone opsin) of center areas of WT mice (C) and 3-month Glast-Cre:Dicer-cKOMG mice (D). (E) Fullfield scotopic ERG recordings showing a-wave and b-wave amplitudes of Glast-Cre:Dicer-cKOMG (n = 6) in comparison to WT (n = 5) and Rlbp-Cre:Dicer-cKOMG (n = 5). (F, F’) Immunofluorescent labeling of central retinal areas of the Glast-Cre:Dicer-cKOMG mouse 6 months after Dicer deletion, with antibodies against RFP (Glast:Tomato), GS, and SOX9, as well as DAPI nuclear staining. Arrows in F show a disturbed ELM, arrowheads in F’ display GS-reduced MG. Scale bars: 50 µm (C, D, F) and 25 µm (F’). Values are expressed as mean ± SEM. Significant differences are indicated. *WT-cKO comparison, +cKO-cKO comparison: P ≤ 0.05, Mann–Whitney U test. Layer explanation is given in Figure 1.
Figure 11.
 
Dicer-depleted MG lead to cone functional loss with subsequent rod loss. Schematic overview of the major events happening in the MG/RPE-driven retinal degeneration model (Rlbp-CreER:Dicer-cKO) and the MG-driven retinal degeneration model (Glast-CreER:Dicer-cKO) emphasizing the shared pathological features: cone outer segment impairment and cone functional loss and hyperreflective foci in the ELM–RPE at the intermediate state and rod degeneration/rod functional loss and interneuron degeneration leading to retinal remodeling at the late stage. Additional features evident in both models include ELM breakage and glial seal formation. The Rlbp-CreER:Dicer-cKO model displayed furthermore impaired RPE contributing to rod loss and accelerating the overall pathological phenotype.
Figure 11.
 
Dicer-depleted MG lead to cone functional loss with subsequent rod loss. Schematic overview of the major events happening in the MG/RPE-driven retinal degeneration model (Rlbp-CreER:Dicer-cKO) and the MG-driven retinal degeneration model (Glast-CreER:Dicer-cKO) emphasizing the shared pathological features: cone outer segment impairment and cone functional loss and hyperreflective foci in the ELM–RPE at the intermediate state and rod degeneration/rod functional loss and interneuron degeneration leading to retinal remodeling at the late stage. Additional features evident in both models include ELM breakage and glial seal formation. The Rlbp-CreER:Dicer-cKO model displayed furthermore impaired RPE contributing to rod loss and accelerating the overall pathological phenotype.
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