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ARVO Annual Meeting Abstract June 2022

cGMP compliant CRISPR correction and retinal differentiation protocol for photoreceptor cell replacement therapy

Laura R Bohrer; Nicholas E Stone; Allison T Wright; Katayoun Varzavand; Melette Devore; Sewoon Han; Todd A Sulchek; Robert F Mullins; Edwin M Stone; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):1356 – F0287. doi:

ARVO Annual Meeting Abstract June 2022

Automated production of patient derived iPSCs for autologous photoreceptor cell replacement.

Budd A. Tucker; Laura R Bohrer; Nicholas E Stone; Kristin R. Anfinson; Cathryn M. Schaffer; Meagan A Luse; Bradley A. Hittle; Kimerly A. Powell; George F. Muschler; Robert F Mullins; Edwin M Stone

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):1860. doi:

ARVO Annual Meeting Abstract June 2022

Characterization of a novel Pde6b -deficient rat model of retinal degeneration and treatment with cGMP-grade AAV vectors.

Luke A Wiley; Ian Han; Katie M. Sheehan; Brynnon E. Harman; Megan J. Riker; Edwin M Stone; Robert F Mullins; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):1783 – F0332. doi:

ARVO Annual Meeting Abstract June 2022

Development of a CRISPR dCas9-KRAB based strategy for treatment for dominant retinal degenerative blindness

Erin R Burnight; Luke A Wiley; Malavika K. Adur; Dalyz Ochoa; Emily E. Kaalberg; Mallory Lang; Jeremy M. Hoffman; Chunhua Jiao; Stephen R Russell; Ian Han; Elliott H Sohn; Jason W. Ross; Robert F Mullins; Edwin M Stone; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):1775 – F0324. doi:

ARVO Annual Meeting Abstract June 2022

Choroidal Neovascularization and Associated Structural Features in Best Vitelliform Macular Dystrophy Using Swept-Source Optical Coherence Tomography Angiography

Ian Han; Razek Georges Coussa; Mahsaw Motlagh; Jonathan Russell; D. Brice Critser; Elliott Sohn; Edwin M Stone

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):2897 – F0050. doi:

ARVO Annual Meeting Abstract June 2022

Development of chemically crosslinked PEG-PAA hydrogels suitable for engineering of the vascularized outer retina

Narendra Pandala; Michael LaScola; Kelly Mulfaul; Edwin M Stone; Robert F Mullins; Budd A. Tucker; Erin Lavik

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):3735 – F0341. doi:

ARVO Annual Meeting Abstract June 2022

Propensity of patient-derived hiPSCs for retinal differentiation

Jessica A Cooke; Andrew P Voigt; Michael A Collingwood; Nicholas Edwin Stone; S Scott Whitmore; Adam P DeLuca; Kristin R. Anfinson; Christopher A Vakulskas; Austin J Reutzel; Heather T Daggett; Jeanean L Andorf; Edwin M Stone; Robert F Mullins; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):3730 – F0336. doi:

ARVO Annual Meeting Abstract June 2022

Novel risk factor gene for POAG identified with GWAS of Ocular Hypertension Treatment Study

John H Fingert; Adam Hedberg-Buenz; Benjamin Roos; Erin Boese; Nathan Sears; Andrew Pouw; Young H Kwon; Wallace Alward; Robert F Mullins; Edwin M Stone; Michael G. Anderson; Mae Gordon; Michael A Kass; Kai Wang; Todd E Scheetz

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):4017 – A0359. doi:

ARVO Annual Meeting Abstract June 2022

Relative abundance of FH and FHR-4 in genotyped human donor eyes: significance for AMD pathogenesis

Kelly Mulfaul; Malia M Collins; Louisa M Affatigato; Edwin M Stone; Budd A. Tucker; Robert F Mullins

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):4615 – F0407. doi:

ARVO Annual Meeting Abstract June 2022

Non-random cellular distribution of the mitochondrial 3243A>G variant in the human retina and choroid and its impact on disease phenotype.

Nathaniel Kevin Mullin; Andrew P Voigt; Miles J Flamme-Wiese; Xiuying Liu; Kelsey L Wieland; Edwin M Stone; Budd A. Tucker; Robert F Mullins

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):2654. doi:

ARVO Annual Meeting Abstract June 2022

Molecular Characterization of Neovascular AMD in Human Eyes using Spatial Transcriptomics and Single Cell RNA Sequencing

Robert F Mullins; Andrew P Voigt; Todd E Scheetz; Nathaniel Kevin Mullin; Miles J Flamme-Wiese; Li-Chun Lin; Edwin M Stone; Ian Han; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):2316. doi:

ARVO Annual Meeting Abstract June 2022

Spectacle: A Platform for Interacting with Over 1.5 Million Single-Cell Expression Profiles from the Eye

Andrew P Voigt; Nicholas D Lessing; Edwin M Stone; Budd A. Tucker; Robert F Mullins; Todd E Scheetz

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):1172 – A0026. doi:

ARVO Annual Meeting Abstract June 2022

Simulating the altered photoreceptor cell topography of progressive retinal dystrophies using OCT images and public data

S Scott Whitmore; Adam P DeLuca; Jeaneen L Andorf; Justine L Cheng; Mahsaw Motlagh; Christopher R Fortenbach; D. Brice Critser; Edwin M Stone; Ian Han

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):197 – F0044. doi:

ARVO Annual Meeting Abstract June 2021

Mutational burden of mt3243G in blood correlates with the age of onset of retinal symptoms.

Nathaniel Kevin Mullin; Kristin R. Anfinson; Louisa M. Affatigato; Jeaneen L. Andorf; Megan J. Riker; Robert F Mullins; Edwin M Stone; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):1518. doi:

ARVO Annual Meeting Abstract June 2021

Single-cell RNA sequencing identifies unique gene expression patterns in the foveal and perifoveal human retina

Andrew P Voigt; Nathaniel Kevin Mullin; S Scott Whitmore; Todd E Scheetz; Edwin M Stone; Budd A. Tucker; Robert F Mullins

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):463. doi:

ARVO Annual Meeting Abstract June 2021

Local CFH synthesis protects choroidal endothelial cells from membrane attack complex formation.

Kelly Mulfaul; Nathaniel Kevin Mullin; Joseph C Giacalone; Arwin Shrestha; Heather T Daggett; Edwin M Stone; Budd A. Tucker; Robert F Mullins

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):2927. doi:

ARVO Annual Meeting Abstract June 2021

Development and biological characterization of a clinical gene transfer vector for the treatment of MAK -associated retinitis pigmentosa.

Budd A. Tucker; Erin R Burnight; Cathryn M Cranston; Jessica L Fick; Austin J Reutzel; Melette R. Devore; Trudi Westfall; C. Anthony Scott; Autumn Marsden; Katherine N Gibson-Corley; Luke A Wiley; Ian Han; Diane Slusarski; Robert F Mullins; Edwin M Stone

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):1485. doi:

ARVO Annual Meeting Abstract June 2021

Developing a CRISPR-mediated safe harbor strategy to correct patient-derived induced pluripotent stem cells

Erin R Burnight; Laura R Bohrer; Jessica A Cooke; Dalyz Ochoa; Christy L. Ralston; Elizabeth L. Geary; Megan A. Luse; Robert F Mullins; Edwin M Stone; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):1473. doi:

ARVO Annual Meeting Abstract June 2021

Evaluating the tropism of chimeric helper-dependent adenoviral vectors for delivery of large genes.

Luke A Wiley; Erin R Burnight; Dalyz Ochoa; Malia M. Collins; Katayoun Varzavand; Edwin M Stone; Robert F Mullins; Budd A. Tucker; Ian Han

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):1198. doi:

ARVO Annual Meeting Abstract June 2021

Pre-clinical toxicity analysis following subretinal transplantation of an iPSC-derived retinal cell graft in immune suppressed rats.

Laura R Bohrer; Ian Han; Jessica A Cooke; Erin R Burnight; Kristin R. Anfinson; Emily K Kaalberg; Mallory J Ulferts; Jeremy M Hoffmann; Kelsey L Wieland; Luke A Wiley; Kristan S Worthington; Katherine N Gibson-Corley; Robert F Mullins; Edwin M Stone; Budd A. Tucker

Invest. Ophthalmol. Vis. Sci.. 2021; 62(8):2707. doi:

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cGMP compliant CRISPR correction and retinal differentiation protocol for photoreceptor cell replacement therapy

Automated production of patient derived iPSCs for autologous photoreceptor cell replacement.

Characterization of a novel Pde6b -deficient rat model of retinal degeneration and treatment with cGMP-grade AAV vectors.

Development of a CRISPR dCas9-KRAB based strategy for treatment for dominant retinal degenerative blindness

Choroidal Neovascularization and Associated Structural Features in Best Vitelliform Macular Dystrophy Using Swept-Source Optical Coherence Tomography Angiography

Development of chemically crosslinked PEG-PAA hydrogels suitable for engineering of the vascularized outer retina

Propensity of patient-derived hiPSCs for retinal differentiation

Novel risk factor gene for POAG identified with GWAS of Ocular Hypertension Treatment Study

Relative abundance of FH and FHR-4 in genotyped human donor eyes: significance for AMD pathogenesis

Non-random cellular distribution of the mitochondrial 3243A>G variant in the human retina and choroid and its impact on disease phenotype.

Molecular Characterization of Neovascular AMD in Human Eyes using Spatial Transcriptomics and Single Cell RNA Sequencing

Spectacle: A Platform for Interacting with Over 1.5 Million Single-Cell Expression Profiles from the Eye

Simulating the altered photoreceptor cell topography of progressive retinal dystrophies using OCT images and public data

Mutational burden of mt3243G in blood correlates with the age of onset of retinal symptoms.

Single-cell RNA sequencing identifies unique gene expression patterns in the foveal and perifoveal human retina

Local CFH synthesis protects choroidal endothelial cells from membrane attack complex formation.

Development and biological characterization of a clinical gene transfer vector for the treatment of MAK -associated retinitis pigmentosa.

Developing a CRISPR-mediated safe harbor strategy to correct patient-derived induced pluripotent stem cells

Evaluating the tropism of chimeric helper-dependent adenoviral vectors for delivery of large genes.

Pre-clinical toxicity analysis following subretinal transplantation of an iPSC-derived retinal cell graft in immune suppressed rats.

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