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ARVO Annual Meeting Abstract June 2022

Comparison of long-term gene expression and protein secretion of non-virally transfected Retinal Pigment Epithelial (RPE) cells from human, pig and rabbit for preclinical efficiency analyses.

Nathan Breuillard; Thais Bascuas; Nina Harmening; Gregg Sealy; Mohit Mohit; Gabriele Thumann; Martina Kropp

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):3173 – F0447. doi:

ARVO Annual Meeting Abstract June 2022

Up-scaling and humanization of retinal organotypic culture as a model for intraocular efficiency and toxicity analyses.

Mohit Mohit; Nathan Breuillard; Nina Harmening; Thais Bascuas Castillo; Alain Conti; Gregg Sealy; Gabriele Thumann; Martina Kropp

Invest. Ophthalmol. Vis. Sci.. 2022; 63(7):3161 – F0435. doi:

ARVO Annual Meeting Abstract July 2018

Challenges in GTMP process validation for a non-viral gene therapy

Martina Kropp; Nina Harmening; Mateusz Kecik; Argyrios Chronopoulos; Alain Conti; Tereza Bautzová; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2018; 59(9):4543. doi:

ARVO Annual Meeting Abstract July 2018

Efficient choroidal neovascularization reduction by Sleeping Beauty Transposon-mediated PEDF gene delivery in rat iris and retinal pigment epithelial cells by using a clinical electroporator

Patricia Fernandez; Sergio Recalde; Maria Hernandez; Laura Garcia-Garcia; Jaione Bezunartea; Juan Roberto Rodriguez; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvak; Martina Kropp; Sandra Johnen; Gabriele Thumann; Alfredo García-Layana

Invest. Ophthalmol. Vis. Sci.. 2018; 59(9):223. doi:

ARVO Annual Meeting Abstract June 2017

Rat Primary Cells transfected with PEDF using the Sleeping Beauty Transposon system are effective in reducing the proangiogenic status in choroidal neovascularization

Sergio Recalde; Maria Hernandez; Patricia Fernandez; Laura García-Garcia; Juan Roberto Rodriguez; Jaione Bezunartea; Maite Moreno; Idoia Belza; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvák; Sandra Johnen; Gabriele Thumann; Alfredo Garcia-Layana

Invest. Ophthalmol. Vis. Sci.. 2017; 58(8):1964. doi:

ARVO Annual Meeting Abstract June 2017

Genetic engineering of freshly isolated primary human pigment epithelial cells by non-viral gene delivery for the treatment of neovascular AMD

Sandra Johnen; Sabine Diarra; Nina Harmening; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvák; Peter Walter; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2017; 58(8):4489. doi:

ARVO Annual Meeting Abstract June 2017

Efficient electrotransfer-mediated transfection of rd10 retinas using the non-viral Sleeping Beauty transposon system

Sabine Diarra; Florian Waschkowski; Aracelys Garcia Moreno; Christine Haselier; Stephan Hesse; Zsuzsanna Izsvák; Zoltán Ivics; Gabriele Thumann; Frank Muller; Wilfried Mokwa; Sandra Johnen; Peter Walter

Invest. Ophthalmol. Vis. Sci.. 2017; 58(8):4537. doi:

ARVO Annual Meeting Abstract September 2016

Efficacy of Rat Primary Cells transfected with Pigment Epithelium-Derived Factor using the Sleeping Beauty Transposon system in choroidal neovascularization.

Maria Hernandez; Laura Garcia-Garcia; Sergio Recalde; Patricia Fernandez; Juan Roberto Rodriguez; Jaione Bezunartea-Bezunartea; Daniel Scherman; Sandra Johnen; Gabriele Thumann; Alfredo Garcia-Layana

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):271. doi:

ARVO Annual Meeting Abstract September 2016

RT-qPCR quantification of mRNAs specific of Pigment Epithelium-Derived Factor (PEDF) and Vascular Endothelial Growth Factor (VEGF) in Rat eyes injected in the subretinal space with Primary Cells transfected with hPEDF.

Sergio Recalde; Laurence Jeanson-LEH; Patricia Fernandez; Maria Hernandez; Laura Garcia-Garcia; Jaione Bezunartea-Bezunartea; Daniel Scherman; Séverine Pouillot; Gabriele Thumann; Alfredo Garcia-Layana

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):281. doi:

ARVO Annual Meeting Abstract September 2016

Human PEDF optimized gene for transposon-based gene therapy to treat age-related macular degeneration

Cécile Prat-Souteyrand; Nina Harmening; Martina Kropp; Gregg Sealy; Zsuzsanna Izsvák; Daniel Scherman; Corinne Marie; Sandra Johnen; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):1172. doi:

ARVO Annual Meeting Abstract September 2016

Results of a biodistribution study of Venus transfected pigment epithelial cells transplanted subretinally in rabbits

Martina Kropp; Argyrios Chronopoulos; Alain Conti; Nina Harmening; Séverine Pouillot; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvák; Pablo Aranda; Verónica Fernández; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):1187. doi:

ARVO Annual Meeting Abstract September 2016

Intravitreal Aflibercept reduces the retinal vessel diameter in patients with diabetic macular edema

Andrea Consigli; Athanasios Papanastasiou; Sayon Roy; Gabriele Thumann; Argyrios Chronopoulos

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):2118. doi:

ARVO Annual Meeting Abstract September 2016

Optimized Non-Viral Transfection of human RPE and IPE cells used for a Gene-Therapeutic Treatment of neovascular AMD

Nina Harmening; Gregg Sealy; Martina Kropp; Corinne Marie; Daniel Scherman; Mattia Roncetti; Pablo Aranda; Verónica Fernández; Sandra Johnen; Zsuzsanna Izsvák; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2016; 57(12):4432. doi:

ARVO Annual Meeting Abstract June 2015

Transposon-Based, Targeted Ex Vivo Gene Therapy to Treat Age-related Macular Degeneration

Gabriele Thumann; Martina Kropp; Nina Harmening; TargetAMD Consortium

Invest. Ophthalmol. Vis. Sci.. 2015; 56(7 ):1820. doi:

ARVO Annual Meeting Abstract June 2015

Biodistribution of Rat Primary Cells transfected with SB100X transposon-mediated Pigment Epithelium-Derived Factor (PEDF) gene in Brown Norway rats after subretinal injection

Maria Hernandez; Laura Garcia-Garcia; Sergio Recalde; Patricia Fernandez-Robredo; Juan Roberto Rodriguez Madoz; Daniel Sherman; Zsuzsanna Izsvák; Sandra Johnen; Gabriele Thumann; Alfredo Garcia Layana

Invest. Ophthalmol. Vis. Sci.. 2015; 56(7 ):1835. doi:

ARVO Annual Meeting Abstract June 2015

pFAR4 miniplasmids in combination with the Sleeping Beauty transposon system allow efficient transfection of freshly isolated iris pigment epithelial cells

Martina Kropp; Nina Harmening; Sandra Johnen; Shuwei Tian; Daniel Scherman; Corinne Marie; Zsuzsanna Izsvák; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2015; 56(7 ):2316. doi:

ARVO Annual Meeting Abstract June 2015

The use of mini-plasmids free of antibiotic resistance markers for a gene therapeutical approach to treat AMD

Nina Harmening; Martina Kropp; Sandra Johnen; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvák; Gabriele Thumann

Invest. Ophthalmol. Vis. Sci.. 2015; 56(7 ):2325. doi:

ARVO Annual Meeting Abstract June 2015

Analysis of Potential Tumorigenicity of PEDF-Transfected Primary Human Retinal Pigment Epithelial Cells Using Soft Agar Assay

Anna Dobias; Corinne Marie; Daniel Scherman; Zsuzsanna Izsvák; Zoltan Ivics; Peter Walter; Gabriele Thumann; Sandra Johnen

Invest. Ophthalmol. Vis. Sci.. 2015; 56(7 ):2348. doi:

ARVO Annual Meeting Abstract April 2014

The Use of Plasmids Free of Antibiotic Resistance Markers for Safe and Efficient Sleeping Beauty-Mediated PEDF Gene Delivery into Primary Pigment Epithelial Cells

Gabriele Thumann; Corinne Marie; Zsuzsanna Izsvák; Daniel Scherman; Peter Walter; Sandra Johnen

Invest. Ophthalmol. Vis. Sci.. 2014; 55(13):1826. doi:

ARVO Annual Meeting Abstract April 2014

Non-Viral Sleeping Beauty-Mediated Transfection of Retinal Pigment Epithelial Cells

Anna Dobias; Mattia Ronchetti; Ruggero Cadossi; Peter Walter; Gabriele Thumann; Sandra Johnen

Invest. Ophthalmol. Vis. Sci.. 2014; 55(13):1827. doi:

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Comparison of long-term gene expression and protein secretion of non-virally transfected Retinal Pigment Epithelial (RPE) cells from human, pig and rabbit for preclinical efficiency analyses.

Up-scaling and humanization of retinal organotypic culture as a model for intraocular efficiency and toxicity analyses.

Challenges in GTMP process validation for a non-viral gene therapy

Efficient choroidal neovascularization reduction by Sleeping Beauty Transposon-mediated PEDF gene delivery in rat iris and retinal pigment epithelial cells by using a clinical electroporator

Rat Primary Cells transfected with PEDF using the Sleeping Beauty Transposon system are effective in reducing the proangiogenic status in choroidal neovascularization

Genetic engineering of freshly isolated primary human pigment epithelial cells by non-viral gene delivery for the treatment of neovascular AMD

Efficient electrotransfer-mediated transfection of rd10 retinas using the non-viral Sleeping Beauty transposon system

Efficacy of Rat Primary Cells transfected with Pigment Epithelium-Derived Factor using the Sleeping Beauty Transposon system in choroidal neovascularization.

RT-qPCR quantification of mRNAs specific of Pigment Epithelium-Derived Factor (PEDF) and Vascular Endothelial Growth Factor (VEGF) in Rat eyes injected in the subretinal space with Primary Cells transfected with hPEDF.

Human PEDF optimized gene for transposon-based gene therapy to treat age-related macular degeneration

Results of a biodistribution study of Venus transfected pigment epithelial cells transplanted subretinally in rabbits

Intravitreal Aflibercept reduces the retinal vessel diameter in patients with diabetic macular edema

Optimized Non-Viral Transfection of human RPE and IPE cells used for a Gene-Therapeutic Treatment of neovascular AMD

Transposon-Based, Targeted Ex Vivo Gene Therapy to Treat Age-related Macular Degeneration

Biodistribution of Rat Primary Cells transfected with SB100X transposon-mediated Pigment Epithelium-Derived Factor (PEDF) gene in Brown Norway rats after subretinal injection

pFAR4 miniplasmids in combination with the Sleeping Beauty transposon system allow efficient transfection of freshly isolated iris pigment epithelial cells

The use of mini-plasmids free of antibiotic resistance markers for a gene therapeutical approach to treat AMD

Analysis of Potential Tumorigenicity of PEDF-Transfected Primary Human Retinal Pigment Epithelial Cells Using Soft Agar Assay

The Use of Plasmids Free of Antibiotic Resistance Markers for Safe and Efficient Sleeping Beauty-Mediated PEDF Gene Delivery into Primary Pigment Epithelial Cells

Non-Viral Sleeping Beauty-Mediated Transfection of Retinal Pigment Epithelial Cells

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